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Open AccessArticle

KCTD15 Protein Expression in Peripheral Blood and Acute Myeloid Leukemia

1
IRCCS SDN, Napoli, Via E. Gianturco 113, 80143 Naples, Italy
2
Department of Pediatric Hematology-Oncology, Santobono-Pausilipon Hospital, 80129 Naples, Italy
3
Institute of Biostructures and Bioimaging, C.N.R., 80134 Napoli, Italy
*
Authors to whom correspondence should be addressed.
Diagnostics 2020, 10(6), 371; https://doi.org/10.3390/diagnostics10060371
Received: 24 April 2020 / Revised: 22 May 2020 / Accepted: 2 June 2020 / Published: 4 June 2020
(This article belongs to the Collection Biomarkers in Medicine)
Leukocytes are major cellular components of the inflammatory and immune response systems. After their generation in the bone marrow from hematopoietic stem cells, they maturate as granulocytes (neutrophils, eosinophils, and basophils), monocytes, and lymphocytes. The abnormal accumulation and proliferation of immature blood cells (blasts) lead to severe and widespread diseases such as leukemia. We have recently shown that KCTD15, a member of the potassium channel tetramerization domain containing protein family (KCTD), is remarkably upregulated in leukemic B-cells. Here, we extend our investigation by monitoring the KCTD15 expression levels in circulating lymphocytes, monocytes, and granulocytes, as well as in leukemia cells. Significant differences in the expression level of KCTD15 were detected in normal lymphocytes, monocytes, and granulocytes. Interestingly, we also found overexpression of the protein following leukemic transformation in the case of myeloid cell lineage. Indeed, KCTD15 was found to be upregulated in K562 and NB4 cells, as well as in HL-60 cell lines. This in vitro finding was corroborated by the analysis of KCTD15 mRNA of acute myeloid leukemia (AML) patients reported in the Microarray Innovations in Leukemia (MILE) dataset. Collectively, the present data open interesting perspectives for understanding the maturation process of leukocytes and for the diagnosis/therapy of acute leukemias. View Full-Text
Keywords: KCTD15; peripheral blood; myeloid cell lines; flow cytometry; biomarker; diagnostics KCTD15; peripheral blood; myeloid cell lines; flow cytometry; biomarker; diagnostics
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Smaldone, G.; Coppola, L.; Incoronato, M.; Parasole, R.; Ripaldi, M.; Vitagliano, L.; Mirabelli, P.; Salvatore, M. KCTD15 Protein Expression in Peripheral Blood and Acute Myeloid Leukemia. Diagnostics 2020, 10, 371.

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