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Open AccessArticle

Evaluation of ViroTrack Sero Zika IgG/IgM, a New Rapid and Quantitative Zika Serological Diagnostic Assay

1
Chang Gung Biotechnology Industry Master and PhD Program, Chang Gung University, Taoyuan 33302, Taiwan
2
BluSense Diagnostics, Fruebjergvej 3, DK-2100 Copenhagen, Denmark
3
Division of Infectious Diseases and Vaccinology, School of Public Health, University of California, Berkeley, CA 94720, USA
4
National Virology Laboratory, National Center for Diagnosis and Reference, Ministry of Health, 107 Managua, Nicaragua
5
Division of Pediatric Infectious Diseases, Department of Pediatrics, Chang Gung Memorial and Children’s Hospital, Linkuo 33305, Taiwan
6
Department of Biomedical Sciences, College of Medicine, Chang Gung University, Taoyuan 33302, Taiwan
*
Author to whom correspondence should be addressed.
Diagnostics 2020, 10(6), 372; https://doi.org/10.3390/diagnostics10060372
Received: 5 May 2020 / Revised: 29 May 2020 / Accepted: 1 June 2020 / Published: 4 June 2020
(This article belongs to the Section Diagnostic Microbiology and Infectious Disease)
Dengue virus (DENV) and Zika virus (ZIKV) belong to the flavivirus genus and are antigenically closely related. They also share the same mosquito vector and can cause similar symptoms upon infection. However, DENV and ZIKV infections lead to different clinical sequelae and treatments; therefore, clinicians need rapid and accurate diagnostics capable of distinguishing between the two diseases. Methods: We employed the immuno-magnetic assay technology on a microfluidic cartridge (ViroTrack Sero Zika IgG/IgM) for diagnosis of ZIKV infection based on the aggregation of magnetic nanoparticles. We carried out three serological studies including samples from the Dominican Republic, USA, and Nicaragua, aimed at detecting ZIKV-specific IgG and IgM using the ViroTrack Sero Zika IgG/IgM test. Results: The seroconversion results were comparable with ZIKV IgG and IgM reactivity measured by the commercial ZIKV ELISA kit. The sensitivity and specificity for both ZIKV IgG and IgM tested by the ViroTrack Sero Zika IgG/IgM was approximately 98% and 93%, respectively. Conclusion: Serological detection of ZIKV infection by the new ViroTrack Sero Zika IgG/IgM test shows promising performance and limited cross-reactivity with DENV. View Full-Text
Keywords: diagnosis; dengue virus; Zika virus; immuno-magnetic assay; ViroTrack Sero Zika IgG/IgM diagnosis; dengue virus; Zika virus; immuno-magnetic assay; ViroTrack Sero Zika IgG/IgM
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Liao, T.; Wang, X.; Donolato, M.; Harris, E.; Cruz, M.M.; Balmaseda, A.; Wang, R.Y. Evaluation of ViroTrack Sero Zika IgG/IgM, a New Rapid and Quantitative Zika Serological Diagnostic Assay. Diagnostics 2020, 10, 372.

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