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Article

Identification and Characterization of Serum microRNAs as Biomarkers for Human Disc Degeneration: An RNA Sequencing Analysis

1
Guangdong Provincial Key Laboratory of Orthopedics and Traumatology, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510000, China
2
AO Research Institute Davos, 7270 Davos, Switzerland
3
The Seventh Affiliated Hospital of Sun Yat-sen University, Shenzhen 518107, China
4
Department of Spine Surgery, Shenzhen People’s Hospital, Shenzhen 518020, China
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Diagnostics 2020, 10(12), 1063; https://doi.org/10.3390/diagnostics10121063
Received: 16 October 2020 / Revised: 1 December 2020 / Accepted: 2 December 2020 / Published: 8 December 2020
(This article belongs to the Section Pathology and Molecular Diagnostics)
Circulating microRNAs (miRNAs) have been associated with various degenerative diseases, including intervertebral disc (IVD) degeneration. Lumbar disc herniation (LDH) often occurs in young patients, although the underlying mechanisms are poorly understood. The aim of this work was to generate RNA deep sequencing data of peripheral blood samples from patients suffering from LDH, identify circulating miRNAs, and analyze them using bioinformatics applications. Serum was collected from 10 patients with LDH (Disc Degeneration Group); 10 patients without LDH served as the Control Group. RNA sequencing analysis identified 73 differential circulating miRNAs (p < 0.05) between the Disc Degeneration Group and Control Group. Gene ontology enrichment analysis (p < 0.05) showed that these differentially expressed miRNAs were associated with extracellular matrix, damage reactions, inflammatory reactions, and regulation of apoptosis. Kyoto Encyclopedia of Genes and Genomes analysis showed that the differentially expressed genes were involved in diverse signaling pathways. The profile of miR-766-3p, miR-6749-3p, and miR-4632-5p serum miRNAs was significantly enriched (p < 0.05) in multiple pathways associated with IVD degeneration. miR-766-3p, miR-6749-3p, and miR-4632-5p signature from serum may serve as a noninvasive diagnostic biomarker for LHD manifestation of IVD degeneration. Furthermore, several dysregulated miRNAs may be involved in the pathogenesis of IVD degeneration. Further study is needed to confirm the functional role of the identified miRNAs. View Full-Text
Keywords: circulating microRNA; intervertebral disc; degeneration; disc herniation; biomarker circulating microRNA; intervertebral disc; degeneration; disc herniation; biomarker
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MDPI and ACS Style

Cui, S.; Zhou, Z.; Liu, X.; Richards, R.G.; Alini, M.; Peng, S.; Liu, S.; Zou, X.; Li, Z.; Grad, S. Identification and Characterization of Serum microRNAs as Biomarkers for Human Disc Degeneration: An RNA Sequencing Analysis. Diagnostics 2020, 10, 1063. https://doi.org/10.3390/diagnostics10121063

AMA Style

Cui S, Zhou Z, Liu X, Richards RG, Alini M, Peng S, Liu S, Zou X, Li Z, Grad S. Identification and Characterization of Serum microRNAs as Biomarkers for Human Disc Degeneration: An RNA Sequencing Analysis. Diagnostics. 2020; 10(12):1063. https://doi.org/10.3390/diagnostics10121063

Chicago/Turabian Style

Cui, Shangbin, Zhiyu Zhou, Xizhe Liu, Robert G. Richards, Mauro Alini, Songlin Peng, Shaoyu Liu, Xuenong Zou, Zhen Li, and Sibylle Grad. 2020. "Identification and Characterization of Serum microRNAs as Biomarkers for Human Disc Degeneration: An RNA Sequencing Analysis" Diagnostics 10, no. 12: 1063. https://doi.org/10.3390/diagnostics10121063

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