Enrichment of Dairy-Type Lamb Diet with Microencapsulated Omega-3 Fish Oil: Effects on Growth, Carcass Quality and Meat Fatty Acids
by
, , , and
Davide De Marzo
1,
Giancarlo Bozzo
2,
Edmondo Ceci
2,
Caterina Losacco
1,
Michela Maria Dimuccio
2
,
Rifat Ullah Khan
3, 
Vito Laudadio
1 and
Vincenzo Tufarelli
1,*
1
Department of Precision and Regenerative Medicine and Jonian Area (DiMePRe-J), Section of Veterinary Science and Animal Production, University of Bari ‘Aldo Moro’, Valenzano, 70010 Bari, Italy
2
Department of Veterinary Medicine, University of Bari ‘Aldo Moro’, Valenzano, 70010 Bari, Italy
3
Faculty of Animal Husbandry and Veterinary Sciences, College of Veterinary Sciences, The University of Agriculture, Peshawar 25000, Pakistan
*
Author to whom correspondence should be addressed.
Life 2023, 13(2), 275; https://doi.org/10.3390/life13020275
Submission received: 19 December 2022
/
Revised: 4 January 2023
/
Accepted: 17 January 2023
/
Published: 18 January 2023
(This article belongs to the Section Animal Science)
Abstract
:The hypothesis that adding omega-3 oil to feedlot pellets will improve the meat’s favourable n-3 PUFA composition was tested in this experiment. Therefore, we evaluated the productive traits and modification of the composition of n-3 PUFA of Longissimus lumborum (LL) muscle in growing lambs supplemented with microencapsulated omega-3 oil (MEOIL) in pelleted total mixed rations (TMR). Thirty six one month old Valle del Belice male lambs (14.04 ± 0.1 kg) were randomly distributed to one of the three dietary treatments (n = 12 lambs each) and provided the supplemented diets up to 14 weeks of age: 1. control (CON) pelleted TMR without omega-3 oil supplementation; 2. omega-3 oil fortified pelleted TMR at 1% (MEOIL1) supplementation; and 3- Omega-3 oil fortified pelleted TMR at 3% (MEOIL3) supplementation. Supplementing MEOIL at both levels in diet positively impacted (p < 0.05) body weight (BW) and feed efficiency. At the end of feeding period, most carcass quality traits did not vary significantly (p > 0.05) among groups, with the exception of carcass dressing and loin yield at both levels of MEOIL. The color and physical traits of LL muscle were affected by MEOIL supplementation (p < 0.05), with no significant change in chemical characteristics. Fatty acids composition of meat in term of linolenic, eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) were significantly (p < 0.05) influenced by both levels of MEOIL. It was concluded that the tested microencapsulated omega-3 oil preparation may be included at 1% in lamb diet for increasing unsaturated fatty acids in meat without any detrimental effects on lamb productivity.
1. Introduction
Light lambs are usually preferred by consumers in the Mediterranean region [1] for their low profile of fatty acids (FA). Many factors affect lamb tissue FA composition, including breed, sex, body weight, environmental condition and feeding management [2,3]. Furthermore, some trans-FAs present in the milk and meat are manufactured in response to bacterial biohydrogenation in the rumen and are garnering attention since they are nutritionally beneficial FAs [4,5]. In order to comply with modern nutritional trends, consumers prefer a balanced and healthful diet, with an emphasis on improved meat quality. Due to its biological benefits, meat high in omega-3 polyunsaturated fatty acids (n-3 PUFA) is considered as an important food item. Dietary n-3 PUFAs consumption prevents decline in immune response, reduces the incidence of cardiac diseases and breast cancer, and promotes brain health and fetus development [6]. For these reasons, studies on n-3 PUFAs are under way. Because the conversion of α-linolenic acid (α-LNA) into eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) in humans is about 5–10% of the total amount [7], their daily dietary intake is considered essential.
By lowering saturated fatty acid (SFA) and raising mono- and polyunsaturated fatty acid (MUFAs and PUFAs, respectively) concentrations in diet, it is possible to enhance the quality of lamb meat. This can lower the chances of plaque formation in the arteries of frequent meat consumers [8,9]. Furthermore, it has been shown that the PUFA content of lamb diets can be increased by providing PUFA-rich plant or animal origin oils [10]. However, few research trials have been done on the impact of feed additive encapsulated oil on the physical growth and carcass quality of lambs [11] and very little information is available on suitable supplemental doses of fish oil in lambs. However, a few studies have been conducted on the effects of encapsulated oil supplementation on growth and carcass traits [11]. The amount and type of fat supplied with diet, in particular, can alter unsaturated fatty acid (UFAs) and FA profiles in the rumen of ruminants [12]. Vegetable oils (from soybean, sunflower and canola) are commonly integrated into the diet of lambs [13], but few research reports are available on the FA profile of meat of lambs fed with fish oil.
As a result, we hypothesized that including a microencapsulated fish oil preparation in the lamb diet would improve meat quality and beneficially modulate its FA profile, resulting in a healthier product for consumers. The aim of this study was to evaluate the effects of microencapsulated oil preparation inclusion on lamb growth, carcass traits, physicochemical characteristics and FA profile of lamb meat.
2. Materials and Methods
2.1. Dietary Treatments and Growth Performance
Total mixed rations (TMR) were formulated according to the nutritional formulation and requirements of growing lambs [14] and the three dietary treatments were as follows: (1) control (CON) pelleted TMR without omega-3 oil supplementation; (2) omega-3 oil fortified pelleted TMR at 1% (MEOIL1) supplementation; and (3) omega-3 oil fortified pelleted TMR at 3% (MEOIL3) supplementation (Table 1). The oil supplement was a protected (microencapsulated) and micro-pearled form of fish oil (Nordos Fat®, produced by Trouw Nutrition Italia S.p.A., Bussolengo, Verona, Italy). The FAs composition of the product is reported in Table 2 [15]. Diets were formulated using standard feed analysis to incorporate about 149 g/kg CP and 11.1 MJ/kg metabolizable energy (DM basis) as suggested by NRC (2007). The TMR was milled to 25 mm and then reduced to steam pellets (8 mm diameter) and incorporated into the daily diet [16,17].
After weaning period (30 ± 2.0 days old), a total of 36 dairy-type Valle del Belice male lambs (14.4 ± 0.1 kg) were equally and randomly distributed into three groups (control and two experimental groups). Lambs were reared in individual pens (2.2 1.0 m) and fed a calculated amount of 500 g of their assigned TMR per day (as-fed basis) and then rising gradually to about 3.5% of live weight [14]. After a seven-day adaption phase, the trial on lambs was initiated until the animals reached 14 weeks. The TMRs were divided into two equal meals and fed to the lambs. To avoid too much intake of dry matter (DMI), which leads to intestinal issues and death, rationed feeding was adopted [8]. Water that was fit for drinking was plentiful. Lamb weight was monitored weekly at 07:00 h before feeding. Each lamb’s daily refusals were used to calculate the DMI. All lambs were inspected by a veterinarian during the feeding trial after receiving an anti-clostridial vaccination and treatment for internal parasites prior to the start of the study.
2.2. Carcass Sampling
After 16 h of fasting, all lambs (14 weeks old) were weighed and then slaughtered according to the established procedures [18]. Lambs were completely bled, peeled, and the internal organs were removed and weighed. The carcass was eviscerated and weighed after the removal of internal organs and tissues. The digestive tract was emptied and weighed. To calculate empty BW, the digestive contents were subtracted from the slaughter weight. Immediately, the hot carcasses were weighed and stored at 4 °C for 24 h. Dressing yield was determined by dividing fasting weight by cold carcass weight [19]. The cold carcass was weighed before sawing the backbone vertically into two symmetrical halves. The right side of the carcasses were further divided into wholesale cuts (shoulder, neck, loin, leg, ribs, brisket) and weight of each cut was recorded separately. Bone, lean and dissectible cuts were removed and weighed.
2.3. Meat Traits
Meat pH of Longissimus lumborum (LL) was recorded immediately after slaughter and repeated after 24 h (designated as pH0 and pH24, respectively) at 4 °C using a pH-meter. On LL muscle, meat tenderness and color were evaluated. The color of meat was evaluated in terms of brightness L*, redness a* and yellowness b* using a spectrophotometer [20]. The degree of meat tenderness was tested using an Instron 1140 apparatus (Instron, High Wycombe, UK) on both raw and cooked samples, through Warner-Bratzler shear (WBS) force. The cut sample was cylindrical in shape and had a 1.27-cm-diameter cut that was perpendicular to the path of the muscle fibers. Meat hardness was calculated using the force deformation curve that was found. Slices of a muscle that were 5 cm thick were taken and weighed both before and after the meat was cooked to a temperature of 75 °C inside a vented oven. In order to ascertain the nutritional makeup of meat, sub-samples of meat muscle were examined for lipids, protein, ash and moisture [21,22]. As previously stated by De Marzo et al. [8], 5 g of meat sample was freeze-dried, then crushed before the FA composition could be determined. The FAs were counted as a percentage of total FAs.
2.4. Statistical Analysis
The general linear model procedure was used to process data under completely randomized design using ANOVA [23]. Tukey’s test was employed to assess treatment mean differences for significant dietary effects. Unless otherwise noted, a p < 0.05 was used to determine significance difference.
3. Results
3.1. Growth Performance
Supplementing the tested levels of MEOIL in the current experiment resulted in improved growth performance. It was observed that the final BW was significantly (p = 0.027) increased in lamb fed 3% of MEOIL compared to the other groups; moreover, compared to the control, lambs fed both MEOIL levels resulted in improved (p = 0.029) FCR (Table 3).
3.2. Carcass Quality and Meat Traits
The slaughtering data, carcass measurements and composition and meat cuts are reported in Table 4. At 14 weeks of age, empty BW was significantly (p = 0.034) increased in lamb group fed MEOIL3 compared to the other groups, whereas carcass dressing percentages (hot and cold) were improved (p = 0.041 and p = 0.046, respectively) in both supplemented groups in comparison to the control diet. Furthermore, supplementing MEOIL at both levels affected positively the loin cut yield (p = 0.043). Carcass measurements and composition were not affected by oil inclusion in the diet. The dietary treatments significantly influenced the lamb meat color (Table 5) in terms of lightness (L*) when fed both oil levels compared to the control (p < 0.032); the same was observed for WBS in cooked meat and for meat cooking loss of lambs fed MEOIL at both levels (Table 5). Chemically, the composition of meat was not influenced by dietary treatments.
3.3. Lamb Meat Fatty Acids
The impact of fish oil supplementation on the FA composition of meat is reported in Table 6. Supplementing MEOIL at both levels led to significantly lower content of myristic acid (p = 0.023) and oleic acid (p = 0.036) compared to unsupplemented group. Conversely, heptadecanoic acid (p = 0.047), linolenic acid (p = 0.023), EPA (p = 0.039) and DHA (p = 0.021) was significantly higher in lambs fed both levels of oil compared to the control diet.
4. Discussion
4.1. Growth Performance and Carcass Characteristics
In the current study, final body weight was significantly higher in MEILO3; however, FCR was similar in the supplemented groups. Dietary changes had no negative impact on the health of lambs. Furthermore, no macroscopic lesions or pathological alterations were identified in the muscles and internal organs of lambs fed the various diets. Ruminant milk and meat have a very different FA composition from the offered nutrients because FAs produced from animal nutrients are processed in the rumen before absorption in the gut; this is why ruminants are referred to as “hetero-lipoid animals” [24]. The equilibrium between FA intake, synthesis, desaturation and esterification essentially determines the FA profile in animal products. The absorbed FAs and subsequent tissue deposition in the duodenum are significantly influenced by rumen digestion [25]. The current findings are consistent with the results of Ferreira et al. [26] and Hernández-Garca et al. [27]. Previously, fish oil concentrations of 10 g/kg or less, physical growth and feed efficiency were unchanged [28,29], while at concentrations of greater than 30 g/kg feed consumption and weight gain decreased [28]. Marinova et al. [28] found that a diet supplemented with 10 g/kg fish oil and 20 g/kg sunflower oil decreased fat percentage in loin cut and increased this in the shoulder. In contrast, Annett et al. [29] found that 35 g/kg fish oil increased the leg’s subcutaneous fat in lambs. Furthermore, due to the fat’s detrimental effects on the rumen microbial population, higher concentrations of fish oil decreased feed intake and, subsequently, average daily gain in ruminants.
4.2. Carcass and Meat Quality
In the current study, meat color (L*) was improved in the supplemented groups. Changes in meat color are usually associated with difference in fat contents in the meat [30]. Ponanmpalam et al. [31] suggested that several factors including PUFAs affect meat color and stability. In the current study, shear force was significantly improved in the supplemented groups. These findings are similar to the previous reports on different sources of PUFA [32,33].
It is generally recognized that meat quality and human health are significantly influenced by the FAs makeup of the meat [34]. Consequently, modification of FAs of meat in productive animals has taken place through feeding in recent years. The fat content of the investigated beef muscle samples did not alter significantly in the current investigation, regardless of diet and this conclusion is consistent with Jaworska et al. [35] in lambs fed 10 g/kg of fish oil in diet. On the other hand, Marinova et al. [28] examined the impact of fish oil in lamb diets as additive and found alterations in the distribution of fat in the carcass. Their research suggests that PUFA may have an impact on lamb meat quality and carcass fatness while, without influencing lamb performance or meat cutting, dietary fish oil raises the fatty acid profile of meat.
4.3. Meat Fatty Acids Composition
The present study on fatty acid profile analysis of lamb groups found that oleic and palmitic levels were the highest of all fatty acids tested. In subcutaneous fat and muscles, oleic acids and palmitic are the most abundant fatty acids. Furthermore, the smaller dose of fish oil had an effect on long-chain n-3 fatty acids. The diet fortification with fish oil enhanced the content of EPA and DHA. Because PUFAs are beneficial for human health, their increased presence in lamb meat demonstrates the crucial influence of n-3 fatty acid inclusion on meat quality in the animals’ diet. The decrease in C18:1 n-9 of LL muscle in the current study could be the cause of higher C18:3 n-3 contents [36]. It was suggested by Bessa et al. [37] that increased PUFA contents in the diet may result in inhibition of enzyme activity required for desaturation of C18:0. Similar observations were reported by Jeronimo et al. [38] that linseed oil effectively improved C18:3 n-3 FA in the muscle tissues. This also indicates that part of the fish oil could escape biohydrogenation and be absorbed in the rumen. Cooking loss in this study was reduced in the supplemented groups compared to the control. In general, cooking loss occurs from 15 to 40%. Meat with lower cooking loss has better meat quality because of less nutrients loss during the cooking. From our results, it can be inferred that addition of encapsulated fish oil has protective effects on cooking loss in lamb meat. Long-chain docosapentaenoic acid was found in meat samples and was more abundant in meat from lambs fed fish oil. Popova et al. [39] discovered that, when lambs were fed 2.5% fish oil in their diet, the concentrations of C16:1 and C18:1 increased, more significantly in the subcutaneous fat over the Longissimus dorsi, while C18:0 decreased. As a consequence of the alterations in the individual fatty acids, the amount of the SFA decreased while the sum of the MUFA increased in the lambs’ fat depots. Although fish oil is an expensive by-product ingredient, its addition lowers the grain consumption and improves the feed efficiency, leading to better partial net revenue per animal. The outcomes were similar to those observed in feedlot feeds for crossbred lambs with 12 g/kg fish oil [27]. Cost will govern inclusion levels because there will be a greater need for fishmeal in the future in industries including aquaculture, livestock feed and the production of pharmaceuticals and cosmetics.
5. Conclusions
The findings corroborate the meat lipid profile by showing that the tested oil preparation can be added at 10 g/kg to the lamb diet without negatively influencing growth traits or meat quality. Consequently, our research supported the idea that adding fish oil to the diets of dairy-type lambs would produce meat with a better functional value. To fully confirm our findings, additional research on the ruminal fermentation pattern, feed digestibility and blood properties is required.
Author Contributions
Conceptualization, D.D.M., V.T. and V.L.; methodology D.D.M., V.T. and V.L.; formal analysis, D.D.M., C.L., M.M.D. and E.C.; investigation, D.D.M., V.T. and V.L.; data curation, V.T., G.B. and V.L.; writing—original draft preparation, D.D.M., V.T. and V.L.; writing—review and editing, V.T., V.L., G.B., E.C. and R.U.K.; visualization, V.T. All authors have read and agreed to the published version of the manuscript.
Funding
This research received no external funding.
Institutional Review Board Statement
The present research was carried out in agreement with the Ethics of EU Directive 2010/63/EU for animal welfare and permitted by the Ethics Institutional Committee (Approval Code: DETO.08/2021) of University of Bari Aldo Moro.
Informed Consent Statement
Not applicable.
Data Availability Statement
Not applicable.
Acknowledgments
The Authors thank all their institutions and the lab technicians involved in the study.
Conflicts of Interest
The authors declare no conflict of interest.
References
- Di Grigoli, A.; Bonanno, A.; Rabie Ashkezary, M.; Laddomada, B.; Alabiso, M.; Vitale, F.; Di Miceli, G. Meat Production from Dairy Breed Lambs Due to Slaughter Age and Feeding Plan Based on Wheat Bran. Animals 2019, 9, 892. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Ponnampalam, E.N.; Knight, M.I.; Moate, P.J.; Jacobs, J.L. An alternative approach for sustainable sheep meat production: Implication for food security. J. Anim. Sci. Biotechnol. 2020, 11, 83. [Google Scholar] [CrossRef] [PubMed]
- Pewan, S.B.; Otto, J.R.; Kinobe, R.T.; Adegboye, O.A.; Malau-Aduli, A.E.O. Nutritional enhancement of health beneficial omega-3 long-chain polyunsaturated fatty acids in the muscle, liver, kidney, and heart of Tattykeel Australian white MARGRA lambs fed pellets fortified with omega-3 oil in a feedlot system. Biology 2021, 10, 912. [Google Scholar] [CrossRef] [PubMed]
- Patra, A.K.; Saxena, J. Exploitation of dietary tannins to improve rumen metabolism and ruminant nutrition. J. Sci. Food Agric. 2011, 91, 24–37. [Google Scholar] [CrossRef] [PubMed]
- Budimir, K.; Mozzon, M.; Toderi, M.; D’Ottavio, P.; Trombetta, M.F. Effect of breed on fatty acid composition of meat and subcutaneous adipose tissue of light lambs. Animals 2020, 10, 535. [Google Scholar] [CrossRef] [Green Version]
- Swanson, D.; Block, R.; Mousa, S.A. Omega-3 fatty acids EPA and DHA: Health benefits throughout life. Adv. Nutr. 2012, 3, 1–7. [Google Scholar] [CrossRef] [Green Version]
- Kralik, G.; Škrtić, Z.; Suchý, P.; Straková, E.; Gajčević, Z. Feeding fish oil and linseed oil to laying hens to increase the n-3 PUFA in egg yolk. Acta Vet. Brno 2008, 77, 561–568. [Google Scholar] [CrossRef]
- De Marzo, D.; Laudadio, V.; Khan, R.U.; Tufarelli, V.; Maiorano, G. Feeding of Camelina sativa Seeds to Light-Type Gentile di Puglia Lambs: Effect on Productive Performance and Muscle Fatty Acid Composition. Anim. Biotechnol. 2022, 1–7. [Google Scholar] [CrossRef]
- Tedone, L.; Giannico, F.; Tufarelli, V.; Laudadio, V.; Selvaggi, M.; De Mastro, G.; Colonna, M.A. Camelina sativa (L. Crantz) Fresh Forage Productive Performance and Quality at Different Vegetative Stages: Effects of Dietary Supplementation in Ionica Goats on Milk Quality. Agriculture 2022, 12, 91. [Google Scholar] [CrossRef]
- Sharifi, M.; Bashtani, M.; Naserian, A.A.; Farhangfar, H.; Rasani, M.; Emami, A. Grape seed oil supplementation in lamb diet: Effect on meat oxidation stability and muscle fatty acids. Ital. J. Anim. Sci. 2019, 18, 1302–1309. [Google Scholar] [CrossRef]
- Nguyen, D.V.; Malau-Aduli, B.S.; Nichols, P.D.; Malau-Aduli, A.E. Growth performance and carcass characteristics of Australian prime lambs supplemented with pellets containing canola oil or flaxseed oil. Anim. Prod. Sci. 2017, 58, 2100–2108. [Google Scholar] [CrossRef]
- Doreau, M.; Ferlay, A. Digestion and utilisation of fatty acids by ruminants. Anim. Feed Sci. Technol. 1994, 45, 379–396. [Google Scholar] [CrossRef]
- Ponnampalam, E.N.; Sinclair, A.J.; Hosking, B.J.; Egan, A.R. Effects of dietary lipid type on muscle fatty acid composition, carcass leanness, and meat toughness in lambs. J. Anim. Sci. 2022, 80, 628–636. [Google Scholar] [CrossRef] [PubMed]
- NRC. Nutrient Requirements of Small Ruminants; The National Academies Press: Washington, DC, USA, 2007. [Google Scholar]
- Husveth, F.; Rozsa, L.; Magyar, L.; Bali, G.; Papocsi, P. N-3 fatty acid enrichment of table eggs by adding a fish oil preparation (Nordos Fat®) to the diet of laying hens. Arch. Für Geflügelkunde 2003, 67, 198–203. [Google Scholar]
- Tufarelli, V.; Introna, M.; Cazzato, E.; Mazzei, D.; Laudadio, V. Suitability of partly destoned exhausted olive cake as by-product feed ingredient for lamb production. J. Anim. Sci. 2013, 91, 872–877. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Tufarelli, V.; Khan, R.U.; Laudadio, V. Feeding of wheat middlings in lamb total mixed rations: Effects on growth performance and carcass traits. Anim. Feed Sci. Technol. 2011, 170, 130–135. [Google Scholar] [CrossRef]
- Sarvar, E.N.; Moeini, M.; Poyanmehr, M.; Mikaeli, E. The effects of docking on growth traits, carcass characteristics and blood biochemical parameters of Sanjabi fat-tailed lambs. Asian-Australas. J. Anim. Sci. 2009, 22, 796–802. [Google Scholar] [CrossRef]
- Maiorano, G.; Wilkanowska, A.; Tavaniello, S.; Di Memmo, D.; De Marzo, D.; Gambacorta, M. Effect of intramuscular injections of DL-α-tocopheryl acetate on growth performance and extracellular matrix of growing lambs. Animal 2015, 9, 2060–2064. [Google Scholar] [CrossRef]
- Šicklep, M.; Čandek-Potokar, M. Pork color measurement as affected by bloom time and measurement location. J. Muscle Foods 2007, 18, 78–87. [Google Scholar]
- Folch, J.; Lees, M.; Sloan-Stanley, G.H. A simple method for the isolation and purification of total lipids from animal tissues. J. Biol. Chem. 1957, 226, 497–509. [Google Scholar] [CrossRef]
- AOAC. Official Methods of Analysis, 17th ed.; Association of Official Analytical Chemists: Arlington, VA, USA, 2000. [Google Scholar]
- SAS Institute. SAS/STAT 9.1.3 User’s Guide; SAS Institute: Cary, NC, USA, 2002–2003. [Google Scholar]
- Dugan, M.; Aldai, N.; Aalhus, J.; Rolland, D.; Kramer, J. Forming beef to provide healthier fatty acid profiles. Can. J. Anim. Sci. 2011, 91, 545–556. [Google Scholar] [CrossRef] [Green Version]
- Białek, M.; Czauderna, M.; Białek, A. Partial replacement of rapeseed oil with fish oil, and dietary antioxidants supplementation affects concentrations of biohydrogenation products and conjugated fatty acids in rumen and selected lamb tissues. Anim. Feed. Sci. Technol. 2018, 241, 63–74. [Google Scholar] [CrossRef]
- Ferreira, E.M.; Pires, A.V.; Susin, I.; Gentil, R.S.; Parente, M.O.M.; Nolli, C.P.; Meneghini, R.C.M.; Mendes, C.Q.; Ribeiro, C.V.D.M. Growth, feed intake, carcass characteristics, and meat fatty acid profile of lambs fed soybean oil partially replaced by fish oil blend. Anim. Feed. Sci. Technol. 2014, 187, 9–18. [Google Scholar] [CrossRef] [Green Version]
- Hernández-García, P.A.; Mendoza-Martínez, G.D.; Sánchez, N.; Martínez-García, J.A.; Plata-Pérez, F.X.; Lara-Bueno, A.; Ferraro, S.M. Effects of increasing dietary concentrations of fish oil on lamb performance, ruminal fermentation, and leptin gene expression in perirenal fat. Rev. Bras. De Zootec. 2017, 46, 521–526. [Google Scholar] [CrossRef] [Green Version]
- Marinova, P.; Popava, T.; Banskalieve, V.; Raicheva, E.; Ignatova, M.; Vasileva, V. Effect of fish oil supplemented diet on the performance, carcass composition and quality in lambs. Bulg. J. Agric. Sci. 2007, 13, 729. [Google Scholar]
- Annett, R.W.; Carson, A.F.; Dawson, L.E.R.; Kilpatrick, D.J. Effects of dam breed and dietary source of n-3 polyunsaturated fatty acids on the growth and carcass characteristics of lambs sourced from hill sheep flocks. Animal 2011, 5, 1023–1035. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Priolo, A.; Micol, D.; Agabriel, J. Effects of grass feeding systems on ruminant meat colour and flavour. A review. Anim. Res. 2011, 50, 185–200. [Google Scholar] [CrossRef] [Green Version]
- Ponnampalam, E.N.; Butler, K.L.; McDonagh, M.B.; Jacobs, J.L.; Hopkins, D.L. Relationship between muscle antioxidant status, forms of iron, polyunsaturated fatty acids and functionality (retail colour) of meat in lambs. Meat Sci. 2012, 90, 297–303. [Google Scholar] [CrossRef]
- Díaz, M.T.; Cañeque, V.; Sánchez, C.I.; Lauzurica, S.; Pérez, C.; Fernández, C.; Álvarez, I.; De la Fuente, J. Nutritional and sensory aspects of light lamb meat enriched in n−3 fatty acids during refrigerated storage. Food Chem. 2011, 124, 147–155. [Google Scholar] [CrossRef]
- Abuelfatah, K.; Zuki, A.B.Z.; Goh, Y.M.; Sazili, A.Q. Effects of Enriching Goat Meat with n-3 Polyunsaturated Fatty Acids on Meat Quality and Stability. Small Rumin. Res. 2016, 136, 36–42. [Google Scholar] [CrossRef]
- Kafantaris, I.; Kotsampasi, B.; Christodoulou, V.; Makri, S.; Stagos, D.; Gerasopoulos, K.; Kouretas, D. Effects of dietary grape pomace supplementation on performance, carcass traits and meat quality of lambs. In Vivo 2018, 32, 807–812. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Jaworska, D.; Czauderna, M.; Przybylski, W.; Rozbicka-Wieczorek, A.J. Sensory quality and chemical composition of meat from lambs fed diets enriched with fish and rapeseed oils, carnosic acid and seleno-compounds. Meat Sci. 2016, 119, 185–192. [Google Scholar] [CrossRef] [PubMed]
- Gao, C.; Gao, D.; Zhang, Q.; Wang, Y.; Gao, A. Performance, meat quality, intramuscular fatty acid profile, rumen characteristics and serum parameters of lambs fed microencapsulated or conventional linseed oil. Czech J. Anim. Sci. 2022, 67, 365–373. [Google Scholar] [CrossRef]
- Bessa, R.J.; Alves, S.P.; Jerónimo, E.; Alfaia, C.M.; Prates, J.A.; Santos-Silva, J. Effect of lipid supplements on ruminal biohydrogenation intermediates and muscle fatty acids in lambs. Eur. J. Lipid Sci. Technol. 2007, 109, 868–878. [Google Scholar] [CrossRef]
- Jerónimo, E.; Alves, S.P.; Dentinho, M.T.P.; Martins, S.V.; Prates, J.A.M.; Vasta, V.; Santos-Silva, J.; Bessa, R.J.B. Effect of grape seed extract, Cistus ladanifer L.; and vegetable oil supplementation on fatty acid composition of abomasal digesta and intramuscular fat of lambs. J. Agric. Food Chem. 2010, 58, 10710–10721. [Google Scholar] [CrossRef]
- Popova, T.; Marinova, P.; Banskalieva, V.; Vasileva, V. Content and fatty acid composition of different fat depots of lambs receiving fish oil supplemented diet. Bulg. J. Agric. Sci. 2008, 14, 100–107. [Google Scholar]
Table 1.
Ingredient and chemical composition of diets fed to lambs.
| Ingredients (% as-Fed Basis) | Diet | ||
|---|---|---|---|
| CON | MEOIL1 | MEOIL3 | |
| Corn | 27.90 | 27.90 | 25.80 |
| Barley | 18.50 | 18.50 | 18.00 |
| Wheat bran | 8.50 | 8.50 | 9.10 |
| Soybean meal, 44% CP | 8.50 | 8.50 | 8.50 |
| Oat hay | 7.50 | 7.50 | 7.50 |
| Wheat middlings | 7.00 | 7.00 | 7.50 |
| Dehydrated beet pulp | 5.00 | 5.00 | 4.50 |
| Soybean hulls | 5.00 | 5.00 | 4.50 |
| Sunflower meal | 4.00 | 4.00 | 4.50 |
| Field bean | 3.50 | 3.50 | 3.50 |
| Calcium carbonate | 2.00 | 2.00 | 2.00 |
| Soybean oil | 1.00 | - | - |
| Microencapsulated oil | - | 1.00 | 3.00 |
| Dicalcium phosphate | 0.50 | 0.50 | 0.50 |
| Sodium chloride | 0.25 | 0.25 | 0.25 |
| Vitamin-mineral premix 1 | 0.25 | 0.25 | 0.25 |
| Calcium sulphate | 0.20 | 0.20 | 0.20 |
| Yeast | 0.20 | 0.20 | 0.20 |
| Magnesium carbonate | 0.10 | 0.10 | 0.10 |
| Sodium bicarbonate | 0.10 | 0.10 | 0.10 |
| Chemical composition (%) | |||
| Dry matter | 89.97 | 89.65 | 89.41 |
| Crude protein | 14.95 | 14.88 | 14.85 |
| Ether extract | 3.50 | 3.54 | 4.95 |
| Neutral detergent fiber | 19.01 | 19.10 | 18.78 |
| Acid detergent fiber | 10.80 | 10.87 | 10.68 |
| Ash | 6.90 | 6.93 | 6.90 |
| Metabolizable energy, MJ/kg DM | 11.02 | 11.05 | 11.17 |
CON = control diet, MEOIL1 = diet containing 1% of microencapsulated oil (Nordos fat®), and MEOIL3 = diet containing 5% of microencapsulated oil (Nordos fat®). 1 Supplied per kg of diet: vitamin A, 13,500 IU; vitamin D3, 2700 IU; vitamin E, 13.5 mg; vitamin B1, 8.44 mg; vitamin B2, 5.06 mg; vitamin B6, 2.02 mg; D-pantothenic acid, 6.75 mg; niacin, 21.93 mg; vitamin B12, 0.01 mg; Co, 0.51 mg; Fe, 67.5 mg; I, 1.65 mg; Mn, 40.5 mg; Se, 0.07 mg; Zn, 101.3 mg.
Table 2.
Fatty acids composition of the microencapsulated oil (Nordos fat®) included in lamb diet 1.
Table 2.
Fatty acids composition of the microencapsulated oil (Nordos fat®) included in lamb diet 1.
| Fatty Acids | % | |
|---|---|---|
| Total lipids | 84.16 | |
| Lauric acid | C12:0 | 0.12 |
| Myristic acid | C14:0 | 3.10 |
| Palmitic acid | C16:0 | 16.12 |
| Palmitoleic acid | C16:1 | 0.48 |
| Stearic acid | C18:0 | 37.72 |
| Oleic acid | C18:1 | 4.64 |
| Linoleic acid | C18:2 n-6 | 2.45 |
| Linolenic acid | C18:3 n-3 | 11.00 |
| Eicosanoic acid | C20:0 | 1.07 |
| Gadoleic acid | C20:1 | 0.21 |
| Dihomo-γ-linolenic acid | C20:3 n-6 | 0.18 |
| Arachidonic acid | C20:4 n-6 | 1.19 |
| Eicosapentaenoic acid | C20:5 n-3 | 5.98 |
| Docosapentaenoic acid | C22:5 n-3 | 2.48 |
| Docosahexaenoic acid | C22:6 n-3 | 11.15 |
| Not identified | - | 2.11 |
| Total fatty acids | 100.00 | |
| Σ SFA | 58.13 | |
| Σ MUFA | 5.33 | |
| Σ n-6 PUFA | 3.82 | |
| Σ n-3 PUFA | 30.61 |
1 Husveth et al. (2003); SFA = saturated fatty acids; MUFA = monounsaturated fatty acids. PUFA = polyunsaturated fatty acids.
Table 3.
Effect of diets on growth performance of lambs fed different levels of microencapsulated oil.
Table 3.
Effect of diets on growth performance of lambs fed different levels of microencapsulated oil.
| Item | Diet | SEM | p-Value | ||
|---|---|---|---|---|---|
| CON | MEOIL1 | MEOIL3 | |||
| Initial BW, kg | 14.4 | 14.5 | 14.4 | 0.276 | 0.898 |
| Final BW, kg | 26.2 b | 26.7 b | 28.8 a | 1.723 | 0.027 |
| Average daily BW gain, g/d | 197 | 203 | 207 | 12.01 | 0.061 |
| Average daily feed intake, g/d | 1153 | 1160 | 1185 | 19.49 | 0.177 |
| Feed conversion ratio, g DM/g gain | 5.85 b | 5.71 a | 5.72 a | 0.198 | 0.029 |
CON = control diet, MEOIL1 = diet containing 1% of microencapsulated oil, MEOIL3 = diet containing 5% of microencapsulated oil. a,b Means within a row with no common superscript differ significantly (p < 0.05).
Table 4.
Effect of dietary treatments on carcass traits of lambs fed different levels of microencapsulated oil.
Table 4.
Effect of dietary treatments on carcass traits of lambs fed different levels of microencapsulated oil.
| Item | Diet | ||||
|---|---|---|---|---|---|
| CON | MEOIL1 | MEOIL3 | SEM | p-Value | |
| Slaughtering data | |||||
| Empty BW, kg | 25.0 b | 25.6 b | 27.3 a | 0.269 | 0.034 |
| Hot carcass dressing 1 | 57.2 b | 58.1 a | 58.5 a | 0.598 | 0.041 |
| Cold carcass dressing 1 | 56.2 b | 57.3 a | 57.4 a | 0.422 | 0.046 |
| Skin 1 | 11.21 | 10.84 | 11.02 | 0.101 | 0.098 |
| Head 1 | 5.13 | 5.34 | 5.57 | 0.087 | 0.111 |
| Offal 1,2 | 4.69 | 5.01 | 5.19 | 0.065 | 0.099 |
| Carcass measurements, cm | |||||
| External carcass length | 75.0 | 76.2 | 75.8 | 0.602 | 0.121 |
| Internal carcass length | 60.5 | 60.7 | 61.0 | 0.430 | 0.098 |
| Leg length | 31.2 | 31.5 | 31.7 | 0.401 | 0.215 |
| Chest width | 21.1 | 21.7 | 21.2 | 0.392 | 0.259 |
| Meat cuts | |||||
| Right-half carcass, kg | 5.24 | 5.79 | 6.12 | 0.067 | 0.098 |
| Neck 3 | 10.49 | 9.78 | 10.15 | 0.125 | 0.087 |
| Shoulder 3 | 17.74 | 18.85 | 18.45 | 0.177 | 0.061 |
| Leg 3 | 31.26 | 31.77 | 31.86 | 0.298 | 0.101 |
| Loin 3 | 8.01 b | 9.84 a | 9.11 a | 0.102 | 0.043 |
| Brisket 3 | 12.05 | 12.44 | 12.50 | 0.163 | 0.091 |
| Ribs 3 | 12.91 | 12.83 | 12.68 | 0.190 | 0.101 |
| Carcass composition | |||||
| Lean 4 | 0.603 | 0.604 | 0.596 | 0.065 | 0.076 |
| Bone 4 | 0.088 | 0.093 | 0.095 | 0.088 | 0.159 |
| Dissectible fat 4 | 0.309 | 0.303 | 0.309 | 0.176 | 0.112 |
CON = control diet, MEOIL1 = diet containing 1% of microencapsulated oil, MEOIL3 = diet containing 5% of microencapsulated oil. 1 Values expressed as % of empty BW. 2 Liver + heart + kidney + lung + spleen. 3 Values expressed as % of right half-carcass. 4 Values expressed as proportion of pelvic limb. a,b Means within a row with no common superscript differ significantly (p < 0.05).
Table 5.
Effect of dietary treatments on physical and chemical parameters of meat muscle (Longissimus lumborum) of lambs fed different levels of microencapsulated oil.
Table 5.
Effect of dietary treatments on physical and chemical parameters of meat muscle (Longissimus lumborum) of lambs fed different levels of microencapsulated oil.
| Item | Diet | ||||
|---|---|---|---|---|---|
| CON | MEOIL1 | MEOIL3 | SEM | p-Value | |
| pH0 | 6.71 | 6.73 | 6.75 | 0.044 | 0.188 |
| pH24 | 5.70 | 5.69 | 5.67 | 0.040 | 0.095 |
| L* | 38.62 b | 40.65 a | 41.91 a | 1.012 | 0.032 |
| a* | 17.52 | 17.13 | 17.09 | 0.165 | 0.082 |
| b* | 7.31 | 7.27 | 7.12 | 0.335 | 0.095 |
| WBS raw, kg/cm2 | 2.97 | 3.13 | 3.66 | 0.149 | 0.078 |
| WBS cooked, kg/cm2 | 3.73 b | 2.69 a | 2.52 a | 0.078 | 0.025 |
| Cooking loss, % | 14.32 b | 13.77 a | 13.85 a | 0.587 | 0.028 |
| Chemical composition, % | |||||
| Moisture | 74.23 | 74.22 | 74.21 | 0.089 | 0.377 |
| Protein | 19.21 | 19.12 | 19.11 | 0.097 | 0.241 |
| Lipids | 5.29 | 5.41 | 5.44 | 0.038 | 0.057 |
| Ash | 1.27 | 1.25 | 1.24 | 0.045 | 0.198 |
CON = control diet, MEOIL1 = diet containing 1% of microencapsulated oil, MEOIL3 = diet containing 5% of microencapsulated oil. pH0 at slaughter; pH24 at 24 h post-mortem; WBS, Warner–Bratzler shear force. a,b Means within a row with no common superscript differ significantly (p < 0.05).
Table 6.
Effect of dietary treatments on meat muscle (Longissimus lumborum) fatty acid composition (% total FA methyl esters) of lambs fed different levels of microencapsulated oil.
Table 6.
Effect of dietary treatments on meat muscle (Longissimus lumborum) fatty acid composition (% total FA methyl esters) of lambs fed different levels of microencapsulated oil.
| Item | Diet | ||||
|---|---|---|---|---|---|
| CON | MEOIL1 | MEOIL3 | SEM | p-Value | |
| C10:0 Capric | 0.33 | 0.13 | 0.21 | 0.077 | 0.052 |
| C12:0 Lauric | 0.21 | 0.16 | 0.22 | 0.022 | 0.104 |
| C14:0 Myristic | 4.73 b | 2.55 a | 3.09 a | 0.917 | 0.023 |
| C15:0 Pentadecylic | 0.56 | 0.47 | 0.58 | 0.070 | 0.211 |
| C16:0 Palmitic | 23.04 | 23.10 | 23.07 | 0.833 | 0.095 |
| C17:0 Heptadecanoic | 1.67 b | 3.16 a | 3.00 a | 0.159 | 0.047 |
| C18:0 Stearic | 18.30 | 18.95 | 18.55 | 0.321 | 0.083 |
| C20:0 Arachidic | 0.16 | 0.21 | 0.20 | 0.011 | 0.065 |
| C16:1 n-7 Palmitoleic | 1.95 | 1.70 | 1.81 | 0.077 | 0.088 |
| C17:1 Heptadecenoic | 0.76 | 0.66 | 0.65 | 0.035 | 0.195 |
| C18:1 n-9 Oleic | 42.31 a | 41.97 b | 41.82 b | 0.612 | 0.036 |
| C18:2 n-6 Linoleic | 3.43 | 3.74 | 3.63 | 0.418 | 0.095 |
| C18:3 n-3 α-Linolenic | 1.39 b | 1.48 a | 1.52 a | 0.031 | 0.023 |
| C20:1 Eicosenoic | 0.17 | 0.18 | 0.16 | 0.012 | 0.216 |
| C20:5 n-3 EPA | 0.58 b | 0.67 a | 0.64 a | 0.045 | 0.039 |
| C22:6 n-3 DHA | 0.42 b | 0.87 a | 0.85 a | 0.060 | 0.021 |
| Σ SFA | 48.90 | 48.73 | 48.92 | 1.003 | 0.061 |
| Σ UFA | 51.10 | 51.27 | 51.08 | 1.122 | 0.075 |
| SFA/UFA | 0.96 | 0.95 | 0.96 | 0.045 | 0.096 |
CON = control diet, MEOIL1 = diet containing 1% of microencapsulated oil, MEOIL3 = diet containing 5% of microencapsulated oil. EPA = eicosapentaenoic acid; DHA = docosahexaenoic acid. SFA = saturated fatty acids; UFA = unsaturated fatty acids. a,b Means within a row with no common superscript differ significantly (p < 0.05).
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De Marzo, D.; Bozzo, G.; Ceci, E.; Losacco, C.; Dimuccio, M.M.; Khan, R.U.; Laudadio, V.; Tufarelli, V. Enrichment of Dairy-Type Lamb Diet with Microencapsulated Omega-3 Fish Oil: Effects on Growth, Carcass Quality and Meat Fatty Acids. Life 2023, 13, 275. https://doi.org/10.3390/life13020275
AMA Style
De Marzo D, Bozzo G, Ceci E, Losacco C, Dimuccio MM, Khan RU, Laudadio V, Tufarelli V. Enrichment of Dairy-Type Lamb Diet with Microencapsulated Omega-3 Fish Oil: Effects on Growth, Carcass Quality and Meat Fatty Acids. Life. 2023; 13(2):275. https://doi.org/10.3390/life13020275
Chicago/Turabian StyleDe Marzo, Davide, Giancarlo Bozzo, Edmondo Ceci, Caterina Losacco, Michela Maria Dimuccio, Rifat Ullah Khan, Vito Laudadio, and Vincenzo Tufarelli. 2023. "Enrichment of Dairy-Type Lamb Diet with Microencapsulated Omega-3 Fish Oil: Effects on Growth, Carcass Quality and Meat Fatty Acids" Life 13, no. 2: 275. https://doi.org/10.3390/life13020275
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