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Article

Over Expression of the Cyanobacterial Pgr5-Homologue Leads to Pseudoreversion in a Gene Coding for a Putative Esterase in Synechocystis 6803

1
Department of Plant and Environmental Sciences, Edmond J. Safra Campus, The Alexander Silberman Institute of Life Sciences, Hebrew University of Jerusalem, Givat Ram, 9190402 Jerusalem, Israel
2
Medical Genetics Institute, Shaare Zedek Medical Center, 9103102 Jerusalem, Israel
3
Institute for Integrative Biology of the Cell (I2BC), Commissariat à l’Energie Atomique et aux Energies Alternatives (CEA) Saclay, Centre National de la Recherche Scientifique (CNRS), Université Paris-Saclay, 91198 Gif-sur-Yvette CEDEX, France
*
Author to whom correspondence should be addressed.
Life 2020, 10(9), 174; https://doi.org/10.3390/life10090174
Received: 21 July 2020 / Revised: 31 August 2020 / Accepted: 1 September 2020 / Published: 3 September 2020
(This article belongs to the Special Issue Cellular and Molecular Strategies in Cyanobacterial Survival)
Pgr5 proteins play a major direct role in cyclic electron flow paths in plants and eukaryotic phytoplankton. The genomes of many cyanobacterial species code for Pgr5-like proteins but their function is still uncertain. Here, we present evidence that supports a link between the Synechocystis sp. PCC6803 Pgr5-like protein and the regulation of intracellular redox balance. The knockout strain, pgr5KO, did not display substantial phenotypic response under our experimental conditions, confirming results obtained in earlier studies. However, the overexpression strain, pgr5OE, accumulated 2.5-fold more chlorophyll than the wild type and displayed increased content of photosystems matching the chlorophyll increase. As a result, electron transfer rates through the photosynthetic apparatus of pgr5OE increased, as did the amount of energy stored as glycogen. While, under photoautotrophic conditions, this metabolic difference had only minor effects, under mixotrophic conditions, pgr5OE cultures collapsed. Interestingly, this specific phenotype of pgr5OE mutants displayed a tendency for reverting, and cultures which previously collapsed in the presence of glucose were now able to survive. DNA sequencing of a pgr5OE strain revealed a second site suppression mutation in slr1916, a putative esterase associated with redox regulation. The phenotype of the slr1916 knockout is very similar to that of the strain reported here and to that of the pmgA regulator knockout. These data demonstrate that, in Synechocystis 6803, there is strong selection against overexpression of the Pgr5-like protein. The pseudoreversion event in a gene involved in redox regulation suggests a connection of the Pgr5-like protein to this network. View Full-Text
Keywords: cyanobacteria; electron transport; photosynthesis; carbon metabolism; redox cyanobacteria; electron transport; photosynthesis; carbon metabolism; redox
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MDPI and ACS Style

Margulis, K.; Zer, H.; Lis, H.; Schoffman, H.; Murik, O.; Shimakawa, G.; Krieger-Liszkay, A.; Keren, N. Over Expression of the Cyanobacterial Pgr5-Homologue Leads to Pseudoreversion in a Gene Coding for a Putative Esterase in Synechocystis 6803. Life 2020, 10, 174. https://doi.org/10.3390/life10090174

AMA Style

Margulis K, Zer H, Lis H, Schoffman H, Murik O, Shimakawa G, Krieger-Liszkay A, Keren N. Over Expression of the Cyanobacterial Pgr5-Homologue Leads to Pseudoreversion in a Gene Coding for a Putative Esterase in Synechocystis 6803. Life. 2020; 10(9):174. https://doi.org/10.3390/life10090174

Chicago/Turabian Style

Margulis, Ketty, Hagit Zer, Hagar Lis, Hanan Schoffman, Omer Murik, Ginga Shimakawa, Anja Krieger-Liszkay, and Nir Keren. 2020. "Over Expression of the Cyanobacterial Pgr5-Homologue Leads to Pseudoreversion in a Gene Coding for a Putative Esterase in Synechocystis 6803" Life 10, no. 9: 174. https://doi.org/10.3390/life10090174

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