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Independent Evolution of Sex Chromosomes in Eublepharid Geckos, A Lineage with Environmental and Genotypic Sex Determination

Department of Ecology, Faculty of Science, Charles University, 12844 Prague, Czech Republic
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Life 2020, 10(12), 342; https://doi.org/10.3390/life10120342
Received: 19 November 2020 / Revised: 4 December 2020 / Accepted: 7 December 2020 / Published: 10 December 2020
(This article belongs to the Section Evolutionary Biology)
Geckos demonstrate a remarkable variability in sex determination systems, but our limited knowledge prohibits accurate conclusions on the evolution of sex determination in this group. Eyelid geckos (Eublepharidae) are of particular interest, as they encompass species with both environmental and genotypic sex determination. We identified for the first time the X-specific gene content in the Yucatán banded gecko, Coleonyx elegans, possessing X1X1X2X2/X1X2Y multiple sex chromosomes by comparative genome coverage analysis between sexes. The X-specific gene content of Coleonyx elegans was revealed to be partially homologous to genomic regions linked to the chicken autosomes 1, 6 and 11. A qPCR-based test was applied to validate a subset of X-specific genes by comparing the difference in gene copy numbers between sexes, and to explore the homology of sex chromosomes across eleven eublepharid, two phyllodactylid and one sphaerodactylid species. Homologous sex chromosomes are shared between Coleonyx elegans and Coleonyx mitratus, two species diverged approximately 34 million years ago, but not with other tested species. As far as we know, the X-specific gene content of Coleonyx elegans / Coleonyx mitratus was never involved in the sex chromosomes of other gecko lineages, indicating that the sex chromosomes in this clade of eublepharid geckos evolved independently. View Full-Text
Keywords: Gekkota; reptiles; DNA-seq; sex chromosomes; sex determination; qPCR Gekkota; reptiles; DNA-seq; sex chromosomes; sex determination; qPCR
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MDPI and ACS Style

Pensabene, E.; Kratochvíl, L.; Rovatsos, M. Independent Evolution of Sex Chromosomes in Eublepharid Geckos, A Lineage with Environmental and Genotypic Sex Determination. Life 2020, 10, 342. https://doi.org/10.3390/life10120342

AMA Style

Pensabene E, Kratochvíl L, Rovatsos M. Independent Evolution of Sex Chromosomes in Eublepharid Geckos, A Lineage with Environmental and Genotypic Sex Determination. Life. 2020; 10(12):342. https://doi.org/10.3390/life10120342

Chicago/Turabian Style

Pensabene, Eleonora; Kratochvíl, Lukáš; Rovatsos, Michail. 2020. "Independent Evolution of Sex Chromosomes in Eublepharid Geckos, A Lineage with Environmental and Genotypic Sex Determination" Life 10, no. 12: 342. https://doi.org/10.3390/life10120342

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