Non-Random Sister Chromatid Segregation in Human Tissue Stem Cells
Abstract
:1. Introduction
2. Materials and Methods
2.1. SACK Expansion and Propagation of Human Hepatic Stem Cell Strains
2.2. Colony Formation Studies
2.3. Colcemid Arrest Assay
2.4. Time-Lapse Microscopy Analyses
2.5. Sister Chromatid Segregation Analyses
2.6. Statistical Analyses
3. Results
3.1. Suppression of Asymmetric Cell Kinetics (SACK) Derivation of Human Hepatic Cell Strain 12(3)
3.2. Tissue Stem Cell Kinetics Properties of Human Hepatic Cell Strain 12(3)
3.3. Detection of Non-Random Sister Chromatid Cosegregation by Human Hepatic Strain 12(3) Cells under Conditions that Promote Asymmetric Self-Renewal Kinetics
4. Discussion
5. Conclusions
6. Patents
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Trial No. | Age (Years) | Gender | Purine | CPD |
---|---|---|---|---|
1 | 6 | M | Control-1 | 24 |
Control-2 | 10 | |||
Xn | 38 | |||
Hx | 29 | |||
Xs | 36 | |||
2 | 14 | F | Control | 10 |
Hx | 26 | |||
3 | 1 | M | Control | 22 |
Xn | 10 | |||
Hx-1 | 30 | |||
Hx-2 | 14 | |||
Xs-1 | 77 | |||
Xs-2 | 21 | |||
4 | 24 | M | Control | 16 |
Xs-1 | 26 | |||
Xs-2 | 13 |
Time-Lapse Culture Condition | ||
---|---|---|
Pedigree Type | Xs-Free | Xs-Supplemented |
Asymmetric | 7% | 9% |
Terminal Cell | 23% | 37% |
Terminal Division | 13% | 11% |
Symmetric | 33% | 17% |
Mixed | 23% | 26% |
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Panchalingam, K.; Jacox, L.; Cappiello, B.D.; Sherley, J.L. Non-Random Sister Chromatid Segregation in Human Tissue Stem Cells. Symmetry 2020, 12, 1868. https://doi.org/10.3390/sym12111868
Panchalingam K, Jacox L, Cappiello BD, Sherley JL. Non-Random Sister Chromatid Segregation in Human Tissue Stem Cells. Symmetry. 2020; 12(11):1868. https://doi.org/10.3390/sym12111868
Chicago/Turabian StylePanchalingam, Krishnanchali, Laura Jacox, Benjamin D. Cappiello, and James L. Sherley. 2020. "Non-Random Sister Chromatid Segregation in Human Tissue Stem Cells" Symmetry 12, no. 11: 1868. https://doi.org/10.3390/sym12111868