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Open AccessArticle

Specificity of Anti-Citrullinated Protein Antibodies in Rheumatoid Arthritis

1
Department of Biomarkers and Autoimmunity, Statens Serum Institut, Artillerivej 5, 2300 Copenhagen S, Denmark
2
Department of Clinical Immunology, Rigshospitalet, Ole Maaløes vej 26, 2200 Copenhagen N, Denmark
3
Department of Drug Design and Pharmacology, Universitetsparken 2, 2100 Copenhagen Ø, Denmark
4
Department of Rheumatology, Rigshospitalet Glostrup, Nordre Ringvej 57, 2600 Glostrup, Denmark
5
Department of Rheumatology, Frederiksberg Hospital, Nordre Fasanvej 57, 2000 Frederiksberg, Denmark
*
Authors to whom correspondence should be addressed.
Antibodies 2019, 8(2), 37; https://doi.org/10.3390/antib8020037
Received: 23 April 2019 / Revised: 28 May 2019 / Accepted: 6 June 2019 / Published: 7 June 2019
(This article belongs to the Special Issue Antibody-Based Diagnostics)
Rheumatoid arthritis (RA) is an autoimmune disease of unknown etiology. The majority of individuals with RA are positive for the disease-specific anti-citrullinated protein antibodies (ACPAs). These antibodies are primarily of cross-reactive nature, hence, the true autoantigen to ACPA remains unidentified. In this study, we analyzed the reactivity of RA sera to several post-translationally modified epitopes, in order to further characterize the specific nature of ACPAs by immunoassays. Substituting citrulline with other amino acids, e.g., D-citrulline, homo-citrulline and methyl-arginine illustrated that ACPAs are utmost specific for citrullinated targets. Collectively, these findings support that ACPAs and citrullinated targets are specific for RA, making citrulline-containing peptide targets the most effective assays for detection of ACPAs. View Full-Text
Keywords: anti-citrullinated protein antibodies; citrulline; homo-citrulline; epitopes; peptides; rheumatoid arthritis anti-citrullinated protein antibodies; citrulline; homo-citrulline; epitopes; peptides; rheumatoid arthritis
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Trier, N.H.; Holm, B.E.; Hansen, P.R.; Slot, O.; Locht, H.; Houen, G. Specificity of Anti-Citrullinated Protein Antibodies in Rheumatoid Arthritis. Antibodies 2019, 8, 37.

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