Codon-Precise, Synthetic, Antibody Fragment Libraries Built Using Automated Hexamer Codon Additions and Validated through Next Generation Sequencing

We have previously described ProxiMAX, a technology that enables the fabrication of precise, combinatorial gene libraries via codon-by-codon saturation mutagenesis. ProxiMAX was originally performed using manual, enzymatic transfer of codons via blunt-end ligation. Here we present Colibra™: an automated, proprietary version of ProxiMAX used specifically for antibody library generation, in which double-codon hexamers are transferred during the saturation cycling process. The reduction in process complexity, resulting library quality and an unprecedented saturation of up to 24 contiguous codons are described. Utility of the method is demonstrated via fabrication of complementarity determining regions (CDR) in antibody fragment libraries and next generation sequencing (NGS) analysis of their quality and diversity. View Full-Text

Keywords: Colibra; ProxiMAX randomization; saturation mutagenesis; CDR; single domain antibody; camelid antibody; antibody engineering

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MDPI and ACS Style

Frigotto, L.; Smith, M.E.; Brankin, C.; Sedani, A.; Cooper, S.E.; Kanwar, N.; Evans, D.; Svobodova, S.; Baar, C.; Glanville, J.; Ullman, C.G.; Hine, A.V. Codon-Precise, Synthetic, Antibody Fragment Libraries Built Using Automated Hexamer Codon Additions and Validated through Next Generation Sequencing. Antibodies 2015, 4, 88-102. https://doi.org/10.3390/antib4020088

AMA Style

Frigotto L, Smith ME, Brankin C, Sedani A, Cooper SE, Kanwar N, Evans D, Svobodova S, Baar C, Glanville J, Ullman CG, Hine AV. Codon-Precise, Synthetic, Antibody Fragment Libraries Built Using Automated Hexamer Codon Additions and Validated through Next Generation Sequencing. Antibodies. 2015; 4(2):88-102. https://doi.org/10.3390/antib4020088

Chicago/Turabian Style

Frigotto, Laura, Matthew E. Smith, Christopher Brankin, Ashni Sedani, Simon E. Cooper, Nisha Kanwar, Daniel Evans, Stanislava Svobodova, Claudia Baar, Jacob Glanville, Christopher G. Ullman, and Anna V. Hine. 2015. "Codon-Precise, Synthetic, Antibody Fragment Libraries Built Using Automated Hexamer Codon Additions and Validated through Next Generation Sequencing" Antibodies 4, no. 2: 88-102. https://doi.org/10.3390/antib4020088

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Codon-Precise, Synthetic, Antibody Fragment Libraries Built Using Automated Hexamer Codon Additions and Validated through Next Generation Sequencing

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