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Open AccessArticle

Comparative Analysis of Bacterial and Archaeal Community Structure in Microwave Pretreated Thermophilic and Mesophilic Anaerobic Digesters Utilizing Mixed Sludge under Organic Overloading

1
UBC Bioreactor Technology Group, School of Engineering, University of British Columbia Okanagan Campus, Kelowna, BC V1V 1V7, Canada
2
Istanbul Technical University, Civil Engineering Faculty, Environmental Engineering Department, 34469 Istanbul, Turkey
*
Author to whom correspondence should be addressed.
Water 2020, 12(3), 887; https://doi.org/10.3390/w12030887 (registering DOI)
Received: 1 March 2020 / Revised: 18 March 2020 / Accepted: 19 March 2020 / Published: 21 March 2020
(This article belongs to the Special Issue Sewage Sludge Treatment and Reuse)
The effects of microwave (MW) pretreatment were investigated by six anaerobic digesters operated under thermophilic and mesophilic conditions at high organic loading rates (4.9–5.7 g volatile solids/L/d). The experiments and analyses were mainly designed to reveal the impact of MW pretreatment and digester temperatures on the process stability and microbial community structure by correlating the composition of microbial populations with volatile fatty acid (VFA) concentrations. A slight shift from biogas production (with a reasonable methane content) to VFA accumulation was observed in the thermophilic digesters, especially in the MW-irradiated reactors. Microbial population structure was assessed using a high-throughput sequencing of 16S rRNA gene on the MiSeq platform. Microbial community structure was slightly affected by different MW pretreatment conditions, while substantially affected by the digester temperature. The phylum Bacteroidetes proliferated in the MW-irradiated mesophilic digesters by resisting high-temperature MW (at 160 °C). Hydrogenotrophic methanogenesis (mostly the genus of Methanothermobacter) was found to be a key route of methane production in the thermophilic digesters, whereas aceticlastic methanogenesis (mostly the genus of Methanosaeta) was the main pathway in the mesophilic digesters. View Full-Text
Keywords: anaerobic digestion; municipal sludge; thermal pretreatment; microwave; microbial community structure; Illumina high-throughput sequencing, volatile fatty acids; bioenergy anaerobic digestion; municipal sludge; thermal pretreatment; microwave; microbial community structure; Illumina high-throughput sequencing, volatile fatty acids; bioenergy
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Kor-Bicakci, G.; Ubay-Cokgor, E.; Eskicioglu, C. Comparative Analysis of Bacterial and Archaeal Community Structure in Microwave Pretreated Thermophilic and Mesophilic Anaerobic Digesters Utilizing Mixed Sludge under Organic Overloading. Water 2020, 12, 887.

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