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Genome-Wide Identification and Expression Profiling of Cytokinin Oxidase/Dehydrogenase (CKX) Genes Reveal Likely Roles in Pod Development and Stress Responses in Oilseed Rape (Brassica napus L.)

1,2,†, 1,2,†, 1,2,†, 1,2, 1,2, 1,2, 1,2, 1,2, 1,2 and 1,2,*
1
Chongqing Rapeseed Engineering Research Center, College of Agronomy and Biotechnology, Southwest University, Chongqing, 400715, China
2
Academy of Agricultural Sciences, Southwest University, Chongqing, 400715, China
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Genes 2018, 9(3), 168; https://doi.org/10.3390/genes9030168
Received: 7 February 2018 / Revised: 10 March 2018 / Accepted: 12 March 2018 / Published: 16 March 2018
(This article belongs to the Section Plant Genetics and Genomics)
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Abstract

Cytokinin oxidase/dehydrogenases (CKXs) play a critical role in the irreversible degradation of cytokinins, thereby regulating plant growth and development. Brassica napus is one of the most widely cultivated oilseed crops worldwide. With the completion of whole-genome sequencing of B. napus, genome-wide identification and expression analysis of the BnCKX gene family has become technically feasible. In this study, we identified 23 BnCKX genes and analyzed their phylogenetic relationships, gene structures, conserved motifs, protein subcellular localizations, and other properties. We also analyzed the expression of the 23 BnCKX genes in the B. napus cultivar Zhong Shuang 11 (‘ZS11’) by quantitative reverse-transcription polymerase chain reaction (qRT-PCR), revealing their diverse expression patterns. We selected four BnCKX genes based on the results of RNA-sequencing and qRT-PCR and compared their expression in cultivated varieties with extremely long versus short siliques. The expression levels of BnCKX5-1, 5-2, 6-1, and 7-1 significantly differed between the two lines and changed during pod development, suggesting they might play roles in determining silique length and in pod development. Finally, we investigated the effects of treatment with the synthetic cytokinin 6-benzylaminopurine (6-BA) and the auxin indole-3-acetic acid (IAA) on the expression of the four selected BnCKX genes. Our results suggest that regulating BnCKX expression is a promising way to enhance the harvest index and stress resistance in plants. View Full-Text
Keywords: Brassica napus; cytokinin oxidase/dehydrogenases (CKX); expression analysis; differentially expressed genes; silique length; harvest index. Brassica napus; cytokinin oxidase/dehydrogenases (CKX); expression analysis; differentially expressed genes; silique length; harvest index.
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Liu, P.; Zhang, C.; Ma, J.-Q.; Zhang, L.-Y.; Yang, B.; Tang, X.-Y.; Huang, L.; Zhou, X.-T.; Lu, K.; Li, J.-N. Genome-Wide Identification and Expression Profiling of Cytokinin Oxidase/Dehydrogenase (CKX) Genes Reveal Likely Roles in Pod Development and Stress Responses in Oilseed Rape (Brassica napus L.). Genes 2018, 9, 168.

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