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Open AccessArticle

Yak IGF2 Promotes Fibroblast Proliferation Via Suppression of IGF1R and PI3KCG Expression

1
College of Life Science and Technology, Gansu Agriculture University, Lanzhou 730070, China
2
College of Veterinary Medicine, Gansu Agriculture University, Lanzhou 730070, China
*
Author to whom correspondence should be addressed.
Genes 2018, 9(3), 169; https://doi.org/10.3390/genes9030169
Received: 30 January 2018 / Revised: 3 March 2018 / Accepted: 12 March 2018 / Published: 20 March 2018
(This article belongs to the Section Animal Genetics and Genomics)
Insulin-like growth factor 2 (IGF2) recapitulates many of the activities of insulin and promotes differentiation of myoblasts and osteoblasts, which likely contribute to genetic variations of growth potential. However, little is known about the functions and signaling properties of IGF2 variants in yaks. The over-expression vector and knockdown sequence of yak IGF2 were transfected into yak fibroblasts, and the effects were detected by a series of assays. IGF2 expression in yak muscle tissues was significantly lower than that of other tissues. In yak fibroblasts, the up-regulated expression of IGF2 inhibits expression of IGF1 and insulin-like growth factor 2 receptor (IGF2R) and significantly up-regulates expression of IGF1R. Inhibition of IGF2 expression caused the up-regulates expression of IGF1, IGF1R and IGF2R. Both over-expression and knockdown of IGF2 resulted in up-regulation of threonine protein kinase 1 (Akt1) expression and down-regulation of phosphatidylinositol 3-kinase, catalytic subunit gamma (PIK3CG). Cell cycle and cell proliferation assays revealed that over-expression of IGF2 enhanced the DNA synthesis phase and promoted yak fibroblasts proliferation. Conversely, knockdown of IGF2 decreased DNA synthesis and inhibited proliferation. These results suggested that IGF2 was negatively correlated with IGF1R and PIK3CG and demonstrated an association with the IGFs-PI3K-Akt (IGFs-phosphatidylinositol 3-kinase- threonine protein kinase) pathway in cell proliferation and provided evidence supporting the functional role of IGF2 for use in improving the production performance of yaks. View Full-Text
Keywords: yak fibroblasts; Insulin-like growth factor 2; Insulin-like growth factor 1 receptor 1; Phosphatidylinositol 3-kinase, catalytic subunit gamma PI3KCG; proliferation yak fibroblasts; Insulin-like growth factor 2; Insulin-like growth factor 1 receptor 1; Phosphatidylinositol 3-kinase, catalytic subunit gamma PI3KCG; proliferation
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MDPI and ACS Style

Zhang, Q.; Wang, Q.; Gong, J.; Du, J.; Zhang, Y.; Zhao, X. Yak IGF2 Promotes Fibroblast Proliferation Via Suppression of IGF1R and PI3KCG Expression. Genes 2018, 9, 169.

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