Comprehensive Transcriptome Profiling and Identification of Potential Genes Responsible for Salt Tolerance in Tall Fescue Leaves under Salinity Stress

Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture and Wuhan Botanical Garden, Chinese Academy of Sciences Wuhan, Wuhan 430074, China

The University of Chinese Academy of Sciences, 19 Yuquan Road, Beijing 100049, China

The Institute of Advanced Studies in Coastal Ecology, Ludong University, Yantai 264000, China

Author to whom correspondence should be addressed.

Genes 2018, 9(10), 466; https://doi.org/10.3390/genes9100466

Received: 31 July 2018 / Revised: 10 September 2018 / Accepted: 14 September 2018 / Published: 21 September 2018

(This article belongs to the Section Plant Genetics and Genomics)

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Abstract

Soil salinity is a serious threat to plant growth and crop productivity. Tall fescue utilization in saline areas is limited by its inferior salt tolerance. Thus, a transcriptome study is a prerequisite for future research aimed at providing deeper insights into the molecular mechanisms of tall fescue salt tolerance as well as molecular breeding. Recent advances in sequencing technology offer a platform to achieve this. Here, Illumina RNA sequencing of tall fescue leaves generated a total of 144,339 raw reads. After de novo assembly, unigenes with a total length of 129,749,938 base pairs were obtained. For functional annotations, the unigenes were aligned to various databases. Further structural analyses revealed 79,352 coding DNA sequences and 13,003 microsatellites distributed across 11,277 unigenes as well as single nucleotide polymorphisms. In total, 1862 unigenes were predicted to encode for 2120 transcription factors among which most were key salt-responsive. We determined differential gene expression and distribution per sample and most genes related to salt tolerance and photosynthesis were upregulated in 48 h vs. 24 h salt treatment. Protein interaction analysis revealed a high interaction of chaperonins and Rubisco proteins in 48 h vs. 24 h salt treatment. The gene expressions were finally validated using quantitative polymerase chain reaction (qPCR), which was coherent with sequencing results. View Full-Text

Keywords: tall fescue; salinity stress; photosynthesis; RNA-sequencing; simple sequence repeats transcription factors

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MDPI and ACS Style

Amombo, E.; Li, X.; Wang, G.; An, S.; Wang, W.; Fu, J. Comprehensive Transcriptome Profiling and Identification of Potential Genes Responsible for Salt Tolerance in Tall Fescue Leaves under Salinity Stress. Genes 2018, 9, 466. https://doi.org/10.3390/genes9100466

AMA Style

Amombo E, Li X, Wang G, An S, Wang W, Fu J. Comprehensive Transcriptome Profiling and Identification of Potential Genes Responsible for Salt Tolerance in Tall Fescue Leaves under Salinity Stress. Genes. 2018; 9(10):466. https://doi.org/10.3390/genes9100466

Chicago/Turabian Style

Amombo, Erick, Xiaoning Li, Guangyang Wang, Shao An, Wei Wang, and Jinmin Fu. 2018. "Comprehensive Transcriptome Profiling and Identification of Potential Genes Responsible for Salt Tolerance in Tall Fescue Leaves under Salinity Stress" Genes 9, no. 10: 466. https://doi.org/10.3390/genes9100466

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Comprehensive Transcriptome Profiling and Identification of Potential Genes Responsible for Salt Tolerance in Tall Fescue Leaves under Salinity Stress

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