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Genes 2015, 6(4), 1230-1241;

Haplotypes and Sequence Variation in the Ovine Adiponectin Gene (ADIPOQ)

Gansu Key Laboratory of Herbivorous Animal Biotechnology, Faculty of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China
Gene-Marker Laboratory, Faculty of Agriculture and Life Sciences, Lincoln University, Lincoln 7647, New Zealand
Authors to whom correspondence should be addressed.
Academic Editor: Paolo Cinelli
Received: 21 August 2015 / Revised: 28 October 2015 / Accepted: 3 November 2015 / Published: 23 November 2015
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The adiponectin gene (ADIPOQ) plays an important role in energy homeostasis. In this study five separate regions (regions 1 to 5) of ovine ADIPOQ were analysed using PCR-SSCP. Four different PCR-SSCP patterns (A1-D1, A2-D2) were detected in region-1 and region-2, respectively, with seven and six SNPs being revealed. In region-3, three different patterns (A3-C3) and three SNPs were observed. Two patterns (A4-B4, A5-B5) and two and one SNPs were observed in region-4 and region-5, respectively. In total, nineteen SNPs were detected, with five of them in the coding region and two (c.46T/C and c.515G/A) putatively resulting in amino acid changes (p.Tyr16His and p.Lys172Arg). In region-1, -2 and -3 of 316 sheep from eight New Zealand breeds, variants A1, A2 and A3 were the most common, although variant frequencies differed in the eight breeds. Across region-1 and region-3, nine haplotypes were identified and haplotypes A1-A3, A1-C3, B1-A3 and B1-C3 were most common. These results indicate that the ADIPOQ gene is polymorphic and suggest that further analysis is required to see if the variation in the gene is associated with animal production traits. View Full-Text
Keywords: adiponectin; ADIPOQ; variation; haplotype; PCR-SSCP; sheep adiponectin; ADIPOQ; variation; haplotype; PCR-SSCP; sheep

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An, Q.-M.; Zhou, H.-T.; Hu, J.; Luo, Y.-Z.; Hickford, J.G.H. Haplotypes and Sequence Variation in the Ovine Adiponectin Gene (ADIPOQ). Genes 2015, 6, 1230-1241.

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