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Article

Comprehensive Transcriptome Analysis of Responses during Cold Stress in Wheat (Triticum aestivum L.)

by
Lei Li
1,2,
Chenglin Han
1,
Jinwei Yang
1,
Zhiqiang Tian
1,2,
Ruyun Jiang
1,
Fei Yang
1,
Kemeng Jiao
1,
Menglei Qi
1,
Lili Liu
1,
Baozhu Zhang
1,
Jishan Niu
1,2,
Yumei Jiang
1,2,
Yongchun Li
1,2 and
Jun Yin
1,2,*
1
National Key Laboratory of Wheat and Maize Crop Science, College of Agronomy, Henan Agricultural University, Zhengzhou 450046, China
2
National Engineering Research Centre for Wheat/Henan Technology Innovation Centre of Wheat, Henan Agricultural University, Zhengzhou 450046, China
*
Author to whom correspondence should be addressed.
Genes 2023, 14(4), 844; https://doi.org/10.3390/genes14040844
Submission received: 21 February 2023 / Revised: 22 March 2023 / Accepted: 29 March 2023 / Published: 31 March 2023
(This article belongs to the Special Issue Molecular Mechanisms of Plant Stress Responses)
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Abstract

Wheat production is often impacted by pre-winter freezing damage and cold spells in later spring. To study the influences of cold stress on wheat seedlings, unstressed Jing 841 was sampled once at the seedling stage, followed by 4 °C stress treatment for 30 days and once every 10 days. A total of 12,926 differentially expressed genes (DEGs) were identified from the transcriptome. K-means cluster analysis found a group of genes related to the glutamate metabolism pathway, and many genes belonging to the bHLH, MYB, NAC, WRKY, and ERF transcription factor families were highly expressed. Starch and sucrose metabolism, glutathione metabolism, and plant hormone signal transduction pathways were found. Weighted Gene Co-Expression Network Analysis (WGCNA) identified several key genes involved in the development of seedlings under cold stress. The cluster tree diagram showed seven different modules marked with different colors. The blue module had the highest correlation coefficient for the samples treated with cold stress for 30 days, and most genes in this module were rich in glutathione metabolism (ko00480). A total of eight DEGs were validated using quantitative real-time PCR. Overall, this study provides new insights into the physiological metabolic pathways and gene changes in a cold stress transcriptome, and it has a potential significance for improving freezing tolerance in wheat.
Keywords: transcriptome; wheat; cold stress; freezing tolerance transcriptome; wheat; cold stress; freezing tolerance

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MDPI and ACS Style

Li, L.; Han, C.; Yang, J.; Tian, Z.; Jiang, R.; Yang, F.; Jiao, K.; Qi, M.; Liu, L.; Zhang, B.; et al. Comprehensive Transcriptome Analysis of Responses during Cold Stress in Wheat (Triticum aestivum L.). Genes 2023, 14, 844. https://doi.org/10.3390/genes14040844

AMA Style

Li L, Han C, Yang J, Tian Z, Jiang R, Yang F, Jiao K, Qi M, Liu L, Zhang B, et al. Comprehensive Transcriptome Analysis of Responses during Cold Stress in Wheat (Triticum aestivum L.). Genes. 2023; 14(4):844. https://doi.org/10.3390/genes14040844

Chicago/Turabian Style

Li, Lei, Chenglin Han, Jinwei Yang, Zhiqiang Tian, Ruyun Jiang, Fei Yang, Kemeng Jiao, Menglei Qi, Lili Liu, Baozhu Zhang, and et al. 2023. "Comprehensive Transcriptome Analysis of Responses during Cold Stress in Wheat (Triticum aestivum L.)" Genes 14, no. 4: 844. https://doi.org/10.3390/genes14040844

APA Style

Li, L., Han, C., Yang, J., Tian, Z., Jiang, R., Yang, F., Jiao, K., Qi, M., Liu, L., Zhang, B., Niu, J., Jiang, Y., Li, Y., & Yin, J. (2023). Comprehensive Transcriptome Analysis of Responses during Cold Stress in Wheat (Triticum aestivum L.). Genes, 14(4), 844. https://doi.org/10.3390/genes14040844

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