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Article

Expression of the Sweet Potato MYB Transcription Factor IbMYB48 Confers Salt and Drought Tolerance in Arabidopsis

by
Hongyuan Zhao
1,
Haoqiang Zhao
1,
Yuanfeng Hu
1,
Shanshan Zhang
1,
Shaozhen He
1,
Huan Zhang
1,
Ning Zhao
1,
Qingchang Liu
1,
Shaopei Gao
1,* and
Hong Zhai
1,2,*
1
Key Laboratory of Sweet Potato Biology and Biotechnology, Ministry of Agriculture and Rural Affairs/Beijing Key Laboratory of Crop Genetic Improvement/Laboratory of Crop Heterosis and Utilization, Ministry of Education, College of Agronomy & Biotechnology, China Agricultural University, Beijing 100193, China
2
Sanya Institute, China Agricultural University, Sanya 572025, China
*
Authors to whom correspondence should be addressed.
Genes 2022, 13(10), 1883; https://doi.org/10.3390/genes13101883
Submission received: 13 September 2022 / Revised: 5 October 2022 / Accepted: 11 October 2022 / Published: 17 October 2022
(This article belongs to the Special Issue Sweet Potato Genetics and Genomics)
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Abstract

Sweet potato (Ipomoea batatas (L.) Lam) is one of the most crucial food crops widely cultivated worldwide. In plants, MYB transcription factors play crucial roles in plant growth, defense regulation, and stress resistance. However, the regulatory mechanism of MYBs in salt and drought response remain poorly studied in sweet potato. By screening a transcriptome database for differentially expressed genes between the sweet potato variety Jingshu 6 and its mutant JS6-5 with high anthocyanin and increased tolerance to salt and drought stresses, we identified a R2R3-MYB gene IbMYB48, for which expression was induced by PEG6000, NaCl, abscisic acid (ABA), methyl jasmonic acid (MeJA), salicylic acid (SA) and H2O2. Particle-mediated transient transformation of onion epidermal cells showed IbMYB48 is localized in the nucleus. Transactivation activity assay in yeast cells revealed that IbMYB48 has transactivation activity, and its active domain is located in the carboxyl (C)-terminal region. Furthermore, expression of IbMYB48 confers enhanced tolerance to salt and drought stresses in transgenic Arabidopsis. The contents of endogenous ABA, JA, and proline in transgenic lines were higher than control, and the activity of superoxide dismutase (SOD) was significantly increased under salt and drought stress conditions. By contrast, the accumulation of malondialdehyde (MDA) and H2O2 were lower. Moreover, genes encoding enzymes involved in ABA biosynthetic pathway, JA biosynthesis and signaling pathway, and reactive oxygen species (ROS) scavenging system were significantly up-regulated in transgenic Arabidopsis under salt or drought stress. Altogether, these results suggest IbMYB48 may be a candidate gene for improvement of abiotic stress tolerance.
Keywords: sweet potato; IbMYB48; MYB transcription factor; salt and drought tolerance sweet potato; IbMYB48; MYB transcription factor; salt and drought tolerance

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MDPI and ACS Style

Zhao, H.; Zhao, H.; Hu, Y.; Zhang, S.; He, S.; Zhang, H.; Zhao, N.; Liu, Q.; Gao, S.; Zhai, H. Expression of the Sweet Potato MYB Transcription Factor IbMYB48 Confers Salt and Drought Tolerance in Arabidopsis. Genes 2022, 13, 1883. https://doi.org/10.3390/genes13101883

AMA Style

Zhao H, Zhao H, Hu Y, Zhang S, He S, Zhang H, Zhao N, Liu Q, Gao S, Zhai H. Expression of the Sweet Potato MYB Transcription Factor IbMYB48 Confers Salt and Drought Tolerance in Arabidopsis. Genes. 2022; 13(10):1883. https://doi.org/10.3390/genes13101883

Chicago/Turabian Style

Zhao, Hongyuan, Haoqiang Zhao, Yuanfeng Hu, Shanshan Zhang, Shaozhen He, Huan Zhang, Ning Zhao, Qingchang Liu, Shaopei Gao, and Hong Zhai. 2022. "Expression of the Sweet Potato MYB Transcription Factor IbMYB48 Confers Salt and Drought Tolerance in Arabidopsis" Genes 13, no. 10: 1883. https://doi.org/10.3390/genes13101883

APA Style

Zhao, H., Zhao, H., Hu, Y., Zhang, S., He, S., Zhang, H., Zhao, N., Liu, Q., Gao, S., & Zhai, H. (2022). Expression of the Sweet Potato MYB Transcription Factor IbMYB48 Confers Salt and Drought Tolerance in Arabidopsis. Genes, 13(10), 1883. https://doi.org/10.3390/genes13101883

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