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Article

IbMYB308, a Sweet Potato R2R3-MYB Gene, Improves Salt Stress Tolerance in Transgenic Tobacco

by
Chong Wang
1,2,†,
Lianjun Wang
2,†,
Jian Lei
2,
Shasha Chai
2,
Xiaojie Jin
2,
Yuyan Zou
2,
Xiaoqiong Sun
3,
Yuqin Mei
2,4,
Xianliang Cheng
2,
Xinsun Yang
2,
Chunhai Jiao
2,* and
Xiaohai Tian
1,*
1
College of Agriculture, Yangtze University, Jingzhou 434025, China
2
Institute of Food Crops, Hubei Academy of Agricultural Sciences, Wuhan 430072, China
3
College of Agriculture and Animal Husbandry, Qinghai University, Xining 810016, China
4
Key Laboratory for Quality Regulation of Tropical Horticultural Plants of Hainan Province, College of Horticulture, Hainan University, Haikou 570228, China
*
Authors to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Genes 2022, 13(8), 1476; https://doi.org/10.3390/genes13081476
Submission received: 28 July 2022 / Revised: 15 August 2022 / Accepted: 17 August 2022 / Published: 18 August 2022
(This article belongs to the Special Issue Sweet Potato Genetics and Genomics)
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Abstract

The MYB (v-myb avian myeloblastosis viral oncogene homolog) transcription factor family plays an important role in plant growth, development, and response to biotic and abiotic stresses. However, the gene functions of MYB transcription factors in sweet potato (Ipomoea batatas (L.) Lam) have not been elucidated. In this study, an MYB transcription factor gene, IbMYB308, was identified and isolated from sweet potato. Multiple sequence alignment showed that IbMYB308 is a typical R2R3-MYB transcription factor. Further, quantitative real-time PCR (qRT-PCR) analysis revealed that IbMYB308 was expressed in root, stem, and, especially, leaf tissues. Moreover, it showed that IbMYB308 had a tissue-specific profile. The experiment also showed that the expression of IbMYB308 was induced by different abiotic stresses (20% PEG-6000, 200 mM NaCl, and 20% H2O2). After a 200 mM NaCl treatment, the expression of several stress-related genes (SOD, POD, APX, and P5CS) was upregulation in transgenic plants, and the CAT activity, POD activity, proline content, and protein content in transgenic tobacco had increased, while MDA content had decreased. In conclusion, this study demonstrated that IbMYB308 could improve salt stress tolerance in transgenic tobacco. These findings lay a foundation for future studies on the R2R3-MYB gene family of sweet potato and suggest that IbMYB308 could potentially be used as an important positive factor in transgenic plant breeding to improve salt stress tolerance in sweet potato plants.
Keywords: IbMYB308; Ipomoea batatas; salt stress; overexpression; tobacco IbMYB308; Ipomoea batatas; salt stress; overexpression; tobacco

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MDPI and ACS Style

Wang, C.; Wang, L.; Lei, J.; Chai, S.; Jin, X.; Zou, Y.; Sun, X.; Mei, Y.; Cheng, X.; Yang, X.; et al. IbMYB308, a Sweet Potato R2R3-MYB Gene, Improves Salt Stress Tolerance in Transgenic Tobacco. Genes 2022, 13, 1476. https://doi.org/10.3390/genes13081476

AMA Style

Wang C, Wang L, Lei J, Chai S, Jin X, Zou Y, Sun X, Mei Y, Cheng X, Yang X, et al. IbMYB308, a Sweet Potato R2R3-MYB Gene, Improves Salt Stress Tolerance in Transgenic Tobacco. Genes. 2022; 13(8):1476. https://doi.org/10.3390/genes13081476

Chicago/Turabian Style

Wang, Chong, Lianjun Wang, Jian Lei, Shasha Chai, Xiaojie Jin, Yuyan Zou, Xiaoqiong Sun, Yuqin Mei, Xianliang Cheng, Xinsun Yang, and et al. 2022. "IbMYB308, a Sweet Potato R2R3-MYB Gene, Improves Salt Stress Tolerance in Transgenic Tobacco" Genes 13, no. 8: 1476. https://doi.org/10.3390/genes13081476

APA Style

Wang, C., Wang, L., Lei, J., Chai, S., Jin, X., Zou, Y., Sun, X., Mei, Y., Cheng, X., Yang, X., Jiao, C., & Tian, X. (2022). IbMYB308, a Sweet Potato R2R3-MYB Gene, Improves Salt Stress Tolerance in Transgenic Tobacco. Genes, 13(8), 1476. https://doi.org/10.3390/genes13081476

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