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10.3390/genes11090954
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Article

VEGFA and NFE2L2 Gene Expression and Regulation by MicroRNAs in Thyroid Papillary Cancer and Colloid Goiter

by
Leonardo P. Stuchi
1,
Márcia Maria U. Castanhole-Nunes
1,
Nathália Maniezzo-Stuchi
2,
Patrícia M. Biselli-Chicote
1,
Tiago Henrique
3,
João Armando Padovani Neto
4,
Dalisio de-Santi Neto
5,
Ana Paula Girol
2,
Erika C. Pavarino
1 and
Eny Maria Goloni-Bertollo
1,*
1
Research Unit in Genetics and Molecular Biology—UPGEM, Faculty of Medicine of São José do Rio Preto—FAMERP, São José do Rio Preto 15090-000, Brazil
2
Padre Albino University Center—UNIFIPA, Catanduva, São Paulo 15809-144, Brazil
3
Laboratory of Molecular Markers and Bioinformatics, Department of Molecular Biology, Faculty of Medicine of São José do Rio Preto —FAMERP, São José do Rio Preto 15090-000, Brazil
4
Department of Otolaryngology and Head and Neck Surgery, Faculty of Medicine of São José do Rio Preto —FAMERP, São José do Rio Preto 15090-000, Brazil
5
Pathological Anatomy Service, Hospital de Base, Foundation Regional Faculty of Medicine of São José do Rio Preto—FUNFARME, São José do Rio Preto 15090-000, Brazil
*
Author to whom correspondence should be addressed.
Genes 2020, 11(9), 954; https://doi.org/10.3390/genes11090954
Submission received: 27 May 2020 / Revised: 6 August 2020 / Accepted: 10 August 2020 / Published: 19 August 2020
(This article belongs to the Special Issue Genetic Perspectives in Thyroid Cancer)
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Abstract

Deregulation of VEGFA (Vascular Endothelial Growth Factor A) and NFE2L2 (Nuclear Factor (Erythroid-derived 2)-Like 2), involved in angiogenesis and oxidative stress, can lead to thyroid cancer progression. MiR-17-5p and miR-612 are possible regulators of these genes and may promote thyroid disorders. In order to evaluate the involvement of VEGFA, NFE2L2, hsa-miR-17-5p, and hsa-miR-612 in thyroid pathology, we examined tissue samples from colloid goiter, papillary thyroid cancer (PTC), and a normal thyroid. We found higher levels of VEGFA and NFE2L2 transcripts and the VEGFA protein in goiter and PTC samples than in normal tissue. In the goiter, miR-612 and miR-17-5p levels were lower than those in PTC. Tumors, despite showing lower VEGFA mRNA expression, presented higher VEGFA protein levels compared to goiter tissue. In addition, NRF2 (Nuclear Related Transcription Factor 2) protein levels in tumors were higher than those in goiter and normal tissues. Inhibition of miR-17-5p resulted in reduced NFE2L2 expression. Overall, both transcript and protein levels of NFE2L2 and VEGFA were elevated in PTC and colloid goiter. Hsa-miR-612 showed differential expression in PTC and colloid goiter, while hsa-miR-17-5p showed differential expression only in colloid goiter, suggesting that hsa-miR-17-5p may be a positive regulator of NFE2L2 expression in PTC.
Keywords: thyroid neoplasms; goiter; vascular endothelial growth factor A; NF-E2-related factor 2; microRNAs thyroid neoplasms; goiter; vascular endothelial growth factor A; NF-E2-related factor 2; microRNAs

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MDPI and ACS Style

Stuchi, L.P.; Castanhole-Nunes, M.M.U.; Maniezzo-Stuchi, N.; Biselli-Chicote, P.M.; Henrique, T.; Padovani Neto, J.A.; de-Santi Neto, D.; Girol, A.P.; Pavarino, E.C.; Goloni-Bertollo, E.M. VEGFA and NFE2L2 Gene Expression and Regulation by MicroRNAs in Thyroid Papillary Cancer and Colloid Goiter. Genes 2020, 11, 954. https://doi.org/10.3390/genes11090954

AMA Style

Stuchi LP, Castanhole-Nunes MMU, Maniezzo-Stuchi N, Biselli-Chicote PM, Henrique T, Padovani Neto JA, de-Santi Neto D, Girol AP, Pavarino EC, Goloni-Bertollo EM. VEGFA and NFE2L2 Gene Expression and Regulation by MicroRNAs in Thyroid Papillary Cancer and Colloid Goiter. Genes. 2020; 11(9):954. https://doi.org/10.3390/genes11090954

Chicago/Turabian Style

Stuchi, Leonardo P., Márcia Maria U. Castanhole-Nunes, Nathália Maniezzo-Stuchi, Patrícia M. Biselli-Chicote, Tiago Henrique, João Armando Padovani Neto, Dalisio de-Santi Neto, Ana Paula Girol, Erika C. Pavarino, and Eny Maria Goloni-Bertollo. 2020. "VEGFA and NFE2L2 Gene Expression and Regulation by MicroRNAs in Thyroid Papillary Cancer and Colloid Goiter" Genes 11, no. 9: 954. https://doi.org/10.3390/genes11090954

APA Style

Stuchi, L. P., Castanhole-Nunes, M. M. U., Maniezzo-Stuchi, N., Biselli-Chicote, P. M., Henrique, T., Padovani Neto, J. A., de-Santi Neto, D., Girol, A. P., Pavarino, E. C., & Goloni-Bertollo, E. M. (2020). VEGFA and NFE2L2 Gene Expression and Regulation by MicroRNAs in Thyroid Papillary Cancer and Colloid Goiter. Genes, 11(9), 954. https://doi.org/10.3390/genes11090954

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