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Article

Cytogenetic Characterization of Seven Novel satDNA Markers in Two Species of Spined Loaches (Cobitis) and Their Clonal Hybrids

1
Laboratory of Fish Genetics, Institute of Animal Physiology and Genetics, Czech Academy of Sciences, 27721 Liběchov, Czech Republic
2
Department of Zoology, Faculty of Science, Charles University in Prague, 128 00 Prague, Czech Republic
3
Institute of Zoology, Academy of Science of Moldova, MD-2028, Academiei 1, 2001 Chisinau, Moldova
4
Department of Biology and Ecology, Faculty of Science, University of Ostrava, Chittussiho 10, 710 00 Ostrava, Czech Republic
*
Author to whom correspondence should be addressed.
Genes 2020, 11(6), 617; https://doi.org/10.3390/genes11060617
Received: 12 May 2020 / Revised: 31 May 2020 / Accepted: 2 June 2020 / Published: 4 June 2020
Interspecific hybridization is a powerful evolutionary force. However, the investigation of hybrids requires the application of methodologies that provide efficient and indubitable identification of both parental subgenomes in hybrid individuals. Repetitive DNA, and especially the satellite DNA sequences (satDNA), can rapidly diverge even between closely related species, hence providing a useful tool for cytogenetic investigations of hybrids. Recent progress in whole-genome sequencing (WGS) offers unprecedented possibilities for the development of new tools for species determination, including identification of species-specific satDNA markers. In this study, we focused on spined loaches (Cobitis, Teleostei), a group of fishes with frequent interspecific hybridization. Using the WGS of one species, C. elongatoides, we identified seven satDNA markers, which were mapped by fluorescence in situ hybridization on mitotic and lampbrush chromosomes of C. elongatoides, C. taenia and their triploid hybrids (C. elongatoides × 2C. taenia). Two of these markers were chromosome-specific in both species, one had centromeric localization in multiple chromosomes and four had variable patterns between tested species. Our study provided a novel set of cytogenetic markers for Cobitis species and demonstrated that NGS-based development of satDNA cytogenetic markers may provide a very efficient and easy tool for the investigation of hybrid genomes, cell ploidy, and karyotype evolution. View Full-Text
Keywords: clonal vertebrates; hybridization; satellite DNA; FISH; mitotic and lampbrush chromosomes clonal vertebrates; hybridization; satellite DNA; FISH; mitotic and lampbrush chromosomes
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MDPI and ACS Style

Marta, A.; Dedukh, D.; Bartoš, O.; Majtánová, Z.; Janko, K. Cytogenetic Characterization of Seven Novel satDNA Markers in Two Species of Spined Loaches (Cobitis) and Their Clonal Hybrids. Genes 2020, 11, 617. https://doi.org/10.3390/genes11060617

AMA Style

Marta A, Dedukh D, Bartoš O, Majtánová Z, Janko K. Cytogenetic Characterization of Seven Novel satDNA Markers in Two Species of Spined Loaches (Cobitis) and Their Clonal Hybrids. Genes. 2020; 11(6):617. https://doi.org/10.3390/genes11060617

Chicago/Turabian Style

Marta, Anatolie, Dmitry Dedukh, Oldřich Bartoš, Zuzana Majtánová, and Karel Janko. 2020. "Cytogenetic Characterization of Seven Novel satDNA Markers in Two Species of Spined Loaches (Cobitis) and Their Clonal Hybrids" Genes 11, no. 6: 617. https://doi.org/10.3390/genes11060617

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