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Open AccessArticle

High-Throughput RNA Sequencing Reveals NDUFC2-AS lncRNA Promotes Adipogenic Differentiation in Chinese Buffalo (Bubalus bubalis L.)

1
College of Life Sciences, Xinyang Normal University, Xinyang, Henan 464000, China
2
School of Agriculture, Ningxia University, Yinchuan, Ningxia 750021, China
*
Author to whom correspondence should be addressed.
Genes 2019, 10(9), 689; https://doi.org/10.3390/genes10090689
Received: 29 July 2019 / Revised: 2 September 2019 / Accepted: 5 September 2019 / Published: 6 September 2019
(This article belongs to the Section Animal Genetics and Genomics)
The buffalo (Bubalus bubalis L.) is prevalent in China and the increasing demand for meat production has changed its role from being a beast of burden to a meat source. The low fat deposition level has become one of the main barriers for its use in meat production. It is urgent to reveal factors involved in fat deposition in buffalo. This study performed RNA sequencing to investigate both long noncoding RNAs (lncRNAs) and mRNAs of adipose tissues in young and adult buffalos. A total of 124 lncRNAs and 2008 mRNAs showed differential expression patterns between young and adult samples. Coexpression analysis and functional enrichment revealed 585 mRNA–lncRNA pairs with potential function in fat deposition. After validation by qRT-PCR, we focused on a lncRNA transcribed from the ubiquinone oxidoreductase subunit C2 (NDUFC2) antisense (AS) strand which showed high correlation with thyroid hormone responsive protein (THRSP). NDUFC2-AS lncRNA is highly expressed in adipose tissue and maturation adipocytes and mainly exists in the nucleus. Functional assays demonstrated that NDUFC2-AS lncRNA promotes adipogenic differentiation by upregulating the expression levels of THRSP and CCAAT enhancer binding protein alpha (C/EBPα) in buffalo. These results indicate that NDUFC2-AS lncRNA promotes fat deposition in buffalo. View Full-Text
Keywords: Bubalus bubalis L.; adipose; RNA sequencing; long noncoding RNAs; adipogenesis Bubalus bubalis L.; adipose; RNA sequencing; long noncoding RNAs; adipogenesis
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Huang, J.; Zheng, Q.; Wang, S.; Wei, X.; Li, F.; Ma, Y. High-Throughput RNA Sequencing Reveals NDUFC2-AS lncRNA Promotes Adipogenic Differentiation in Chinese Buffalo (Bubalus bubalis L.). Genes 2019, 10, 689.

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