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Immunophenotyping of a Stromal Vascular Fraction from Microfragmented Lipoaspirate Used in Osteoarthritis Cartilage Treatment and Its Lipoaspirate Counterpart

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Srebrnjak Children’s Hospital, HR-10000 Zagreb, Croatia
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Specialty Hospital St. Catherine, HR-49120 Zabok, Croatia
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Clinical Hospital Sveti Duh, HR-10000 Zagreb, Croatia
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School of Medicine, Josip Juraj Strossmayer University of Osijek, HR-31000 Osijek, Croatia
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School of Medicine, University of Rijeka, HR-51000 Rijeka, Croatia
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School of Medicine, University of Split, HR-21000 Split, Croatia
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Genos Glycoscience Research Laboratory, HR-10000 Zagreb, Croatia
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Catholic University of Croatia, HR-10000 Zagreb, Croatia
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University of New Haven, West Haven, CT 06516, USA
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Department of Biotechnology, University of Rijeka, HR-51000 Rijeka, Croatia
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Eberly College of Science, The Pennsylvania State University, University Park, PA 16803, USA
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Authors to whom correspondence should be addressed.
Genes 2019, 10(6), 474; https://doi.org/10.3390/genes10060474
Received: 30 April 2019 / Revised: 18 June 2019 / Accepted: 18 June 2019 / Published: 21 June 2019
(This article belongs to the Special Issue Stem Cells Application in Clinical Practice: Advances and Challenges)
Osteoarthritis (OA) is a degenerative joint disease accompanied by pain and loss of function. Adipose tissue harbors mesenchymal stem/stromal cells (MSC), or medicinal signaling cells as suggested by Caplan (Caplan, 2017), used in autologous transplantation in many clinical settings. The aim of the study was to characterize a stromal vascular fraction from microfragmented lipoaspirate (SVF-MLA) applied for cartilage treatment in OA and compare it to that of autologous lipoaspirate (SVF-LA). Samples were first stained using a DuraClone SC prototype tube for the surface detection of CD31, CD34, CD45, CD73, CD90, CD105, CD146 and LIVE/DEAD Yellow Fixable Stain for dead cell detection, followed by DRAQ7 cell nuclear dye staining, and analyzed by flow cytometry. In SVF-LA and SVF-MLA samples, the following population phenotypes were identified within the CD45 fraction: CD31+CD34+CD73±CD90±CD105±CD146± endothelial progenitors (EP), CD31+CD34CD73±CD90±CD105CD146± mature endothelial cells, CD31CD34CD73±CD90+CD105CD146+ pericytes, CD31CD34+CD73±CD90+CD105CD146+ transitional pericytes, and CD31CD34+CD73highCD90+CD105CD146 supra-adventitial-adipose stromal cells (SA-ASC). The immunophenotyping profile of SVF-MLA was dominated by a reduction of leukocytes and SA-ASC, and an increase in EP, evidencing a marked enrichment of this cell population in the course of adipose tissue microfragmentation. The role of EP in pericyte-primed MSC-mediated tissue healing, as well as the observed hormonal implication, is yet to be investigated. View Full-Text
Keywords: lipoaspirate; microfragmented lipoaspirate; immunophenotyping; endothelial progenitors; pericytes; supra-adventitial adipose stromal cells lipoaspirate; microfragmented lipoaspirate; immunophenotyping; endothelial progenitors; pericytes; supra-adventitial adipose stromal cells
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Polancec, D.; Zenic, L.; Hudetz, D.; Boric, I.; Jelec, Z.; Rod, E.; Vrdoljak, T.; Skelin, A.; Plecko, M.; Turkalj, M.; Nogalo, B.; Primorac, D. Immunophenotyping of a Stromal Vascular Fraction from Microfragmented Lipoaspirate Used in Osteoarthritis Cartilage Treatment and Its Lipoaspirate Counterpart. Genes 2019, 10, 474.

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