Bacterial Aspartyl-tRNA Synthetase Has Glutamyl-tRNA Synthetase Activity
AbstractThe aminoacyl-tRNA synthetases (aaRSs) are well established as the translators of the genetic code, because their products, the aminoacyl-tRNAs, read codons to translate messenger RNAs into proteins. Consequently, deleterious errors by the aaRSs can be transferred into the proteome via misacylated tRNAs. Nevertheless, many microorganisms use an indirect pathway to produce Asn-tRNAAsn via Asp-tRNAAsn. This intermediate is produced by a non-discriminating aspartyl-tRNA synthetase (ND-AspRS) that has retained its ability to also generate Asp-tRNAAsp. Here we report the discovery that ND-AspRS and its discriminating counterpart, AspRS, are also capable of specifically producing Glu-tRNAGlu, without producing misacylated tRNAs like Glu-tRNAAsn, Glu-tRNAAsp, or Asp-tRNAGlu, thus maintaining the fidelity of the genetic code. Consequently, bacterial AspRSs have glutamyl-tRNA synthetase-like activity that does not contaminate the proteome via amino acid misincorporation. View Full-Text
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Rathnayake, U.M.; Hendrickson, T.L. Bacterial Aspartyl-tRNA Synthetase Has Glutamyl-tRNA Synthetase Activity. Genes 2019, 10, 262.
Rathnayake UM, Hendrickson TL. Bacterial Aspartyl-tRNA Synthetase Has Glutamyl-tRNA Synthetase Activity. Genes. 2019; 10(4):262.Chicago/Turabian Style
Rathnayake, Udumbara M.; Hendrickson, Tamara L. 2019. "Bacterial Aspartyl-tRNA Synthetase Has Glutamyl-tRNA Synthetase Activity." Genes 10, no. 4: 262.
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