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Genes 2019, 10(4), 262; https://doi.org/10.3390/genes10040262

Bacterial Aspartyl-tRNA Synthetase Has Glutamyl-tRNA Synthetase Activity

Department of Chemistry, Wayne State University, 5101 Cass Avenue, Detroit, MI 48202, USA
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Received: 14 March 2019 / Revised: 25 March 2019 / Accepted: 27 March 2019 / Published: 1 April 2019
(This article belongs to the Special Issue Synthetic DNA and RNA Programming)
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Abstract

The aminoacyl-tRNA synthetases (aaRSs) are well established as the translators of the genetic code, because their products, the aminoacyl-tRNAs, read codons to translate messenger RNAs into proteins. Consequently, deleterious errors by the aaRSs can be transferred into the proteome via misacylated tRNAs. Nevertheless, many microorganisms use an indirect pathway to produce Asn-tRNAAsn via Asp-tRNAAsn. This intermediate is produced by a non-discriminating aspartyl-tRNA synthetase (ND-AspRS) that has retained its ability to also generate Asp-tRNAAsp. Here we report the discovery that ND-AspRS and its discriminating counterpart, AspRS, are also capable of specifically producing Glu-tRNAGlu, without producing misacylated tRNAs like Glu-tRNAAsn, Glu-tRNAAsp, or Asp-tRNAGlu, thus maintaining the fidelity of the genetic code. Consequently, bacterial AspRSs have glutamyl-tRNA synthetase-like activity that does not contaminate the proteome via amino acid misincorporation. View Full-Text
Keywords: tRNA; misacylation; indirect tRNA aminoacylation; AspRS; GluRS-like tRNA; misacylation; indirect tRNA aminoacylation; AspRS; GluRS-like
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Rathnayake, U.M.; Hendrickson, T.L. Bacterial Aspartyl-tRNA Synthetase Has Glutamyl-tRNA Synthetase Activity. Genes 2019, 10, 262.

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