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Open AccessArticle

MicroRNA-1258 Inhibits the Proliferation and Migration of Human Colorectal Cancer Cells through Suppressing CKS1B Expression

1
Biotherapeutics Translational Research Center, Division of Biomedical Science, Korea Research Institute of Bioscience and Biotechnology, Daejeon 34141, Korea
2
Department of Functional Genomics, University of Science and Technology, Daejeon 34141, Korea
3
Department of Biological Science, College of Natural Sciences, Wonkwang University, Iksan 570-450, Korea
4
Personalized Genomic Medicine Research Center, Division of Biomedical Science, Korea Research Institute of Bioscience and Biotechnology, Daejeon 34141, Korea
*
Authors to whom correspondence should be addressed.
Genes 2019, 10(11), 912; https://doi.org/10.3390/genes10110912
Received: 14 October 2019 / Revised: 1 November 2019 / Accepted: 6 November 2019 / Published: 8 November 2019
(This article belongs to the Special Issue Non-coding RNAs: Variety of Roles and Applications in Human Diseases)
Increasing evidence has demonstrated that increased expression of cyclin-dependent kinase regulatory subunit 1B (CKS1B) is associated with the pathogenesis of many human cancers, including colorectal cancer (CRC). However, the regulatory mechanisms underlying the expression of CKS1B in CRC are not completely understood. Here, we investigate the role played by microRNAs in the expression of CKS1B and carcinogenesis in CRC. Among the six microRNAs predicted to target CKS1B gene expression, only miR-1258 was revealed to downregulate CKS1B expression through binding to its 3’-UTR region, as ectopic miR-1258 expression suppressed CKS1B expression and vice versa. In CRC, miR-1258 expression also decreased cell proliferation and migration in vitro and tumor growth in vivo, similar to cells with silenced CKS1B expression. Considering the highly increased levels of CKS1B and decreased expression of miR-1258 in tumors from CRC patients, these findings suggest that miR-1258 may play tumor-suppressive roles by targeting CKS1B expression in CRC. However, the therapeutic significance of these findings should be evaluated in clinical settings.
Keywords: colorectal cancer; CKS1B; miR-1258 colorectal cancer; CKS1B; miR-1258
MDPI and ACS Style

Hwang, J.-S.; Jeong, E.-J.; Choi, J.; Lee, Y.-J.; Jung, E.; Kim, S.-K.; Min, J.-K.; Han, T.-S.; Kim, J.-S. MicroRNA-1258 Inhibits the Proliferation and Migration of Human Colorectal Cancer Cells through Suppressing CKS1B Expression. Genes 2019, 10, 912.

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