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Open AccessArticle

Genome-Wide Analysis of Members of the WRKY Gene Family and Their Cold Stress Response in Prunus mume

by Fei Bao 1,2,3,4,5, Anqi Ding 1,2,3,4,5,6, Tangren Cheng 2,3,4,5, Jia Wang 2,3,4,5 and Qixiang Zhang 1,2,3,4,5,6,*
1
Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing Forestry University, Beijing 100083, China
2
Beijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding, Beijing Forestry University, Beijing 100083, China
3
National Engineering Research Center for Floriculture, Beijing Forestry University, Beijing 100083, China
4
Beijing Laboratory of Urban and Rural Ecological Environment, Beijing Forestry University, Beijing 100083, China
5
Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education, Beijing Forestry University, Beijing 100083, China
6
School of Landscape Architecture, Beijing Forestry University, Beijing 100083, China
*
Author to whom correspondence should be addressed.
Genes 2019, 10(11), 911; https://doi.org/10.3390/genes10110911
Received: 14 October 2019 / Revised: 5 November 2019 / Accepted: 6 November 2019 / Published: 8 November 2019
Prunus mume, which is a rosaceous arbor with very high ornamental, edible and medical values, has a distribution that is mainly restricted by low temperature. WRKY transcription factor genes play crucial roles in the growth, development, and stress responses of plants. However, the WRKY gene family has not been characterised in P. mume. There were 58 PmWRKYs identified from genome of P. mume. They were anchored onto eight link groups and categorised into three broad groups. The gene structure and motif composition were reasonably conservative in each group. Investigation of gene duplication indicated that nine and seven PmWRKYs were arranged in tandem and segmental duplications, respectively. PmWRKYs were discriminately expressed in different tissues (i.e., roots, stems, leaves, flowers and fruits) in P. mume. The 17 cold-related candidate genes were selected based on RNA-seq data. Further, to investigate the function of PmWRKYs in low temperatures, the expression patterns under artificial cold treatments were analysed. The results showed that the expression levels of the 12 PmWRKYs genes significantly and 5 genes slightly changed in stems. In particular, the expression level of PmWRKY18 was up-regulated after ABA treatment. In addition, the spatiotemporal expression patterns of 17 PmWRKYs were analysed in winter. These results indicated that 17 PmWRKYs were potential transcription factors regulating cold resistance in P. mume.
Keywords: Prunus mume; genome; WRKY genes; cold response Prunus mume; genome; WRKY genes; cold response
MDPI and ACS Style

Bao, F.; Ding, A.; Cheng, T.; Wang, J.; Zhang, Q. Genome-Wide Analysis of Members of the WRKY Gene Family and Their Cold Stress Response in Prunus mume. Genes 2019, 10, 911.

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