Next Article in Journal
Genetic Diversity Assessed by Genotyping by Sequencing (GBS) in Watermelon Germplasm
Next Article in Special Issue
Frameshift Variant in MFSD12 Explains the Mushroom Coat Color Dilution in Shetland Ponies
Previous Article in Journal
The Complete Mitogenome of Pyrrhocoris tibialis (Hemiptera: Pyrrhocoridae) and Phylogenetic Implications
Previous Article in Special Issue
TRIM39-RPP21 Variants (∆19InsCCC) Are Not Associated with Juvenile Idiopathic Epilepsy in Egyptian Arabian Horses
Open AccessArticle

Non-Coding RNA Sequencing of Equine Endometrium During Maternal Recognition of Pregnancy

1
Department of Animal Sciences, Colorado State University, Fort Collins, CO 80523, USA
2
Department of Statistics and Bioinformatics, Colorado State University, Fort Collins, CO 80523, USA
3
Department of Animal Science, University of California Davis, Davis, CA 95616, USA
4
Department of Veterinary Science, Gluck Equine Research Center, University of Kentucky, Lexington, KY 40503, USA
5
Department of Biomedical Sciences, Animal Reproduction and Biotechnology Laboratory, Colorado State University, Fort Collins, CO 80523, USA
6
Department of Animal Sciences, 259 Animal Sciences, 1171 Campus Delivery Colorado State University, Fort Collins, CO 80523, USA
*
Author to whom correspondence should be addressed.
Genes 2019, 10(10), 821; https://doi.org/10.3390/genes10100821
Received: 6 August 2019 / Revised: 4 October 2019 / Accepted: 10 October 2019 / Published: 18 October 2019
(This article belongs to the Special Issue Equine Genetics)
Maternal recognition of pregnancy (MRP) in the mare is not well defined. In a non-pregnant mare, prostaglandin F (PGF) is released on day 14 post-ovulation (PO) to cause luteal regression, resulting in loss of progesterone production. Equine MRP occurs prior to day 14 to halt PGF production. Studies have failed to identify a gene candidate for MRP, so attention has turned to small, non-coding RNAs. The objective of this study was to evaluate small RNA (<200 nucleotides) content in endometrium during MRP. Mares were used in a cross-over design with each having a pregnant and non-mated cycle. Each mare was randomly assigned to collection day 11 or 13 PO (n = 3/day) and endometrial biopsies were obtained. Total RNA was isolated and sequencing libraries were prepared using a small RNA library preparation kit and sequenced on a HiSeq 2000. EquCab3 was used as the reference genome and DESeq2 was used for statistical analysis. On day 11, 419 ncRNAs, representing miRNA, snRNA, snoRNA, scaRNA, and vaultRNA, were different between pregnancy statuses, but none on day 13. Equine endometrial ncRNAs with unknown structure and function were also identified. This study is the first to describe ncRNA transcriptome in equine endometrium. Identifying targets of these ncRNAs could lead to determining MRP. View Full-Text
Keywords: equine; pregnancy; maternal recognition of pregnancy; embryo; non-coding RNA; endometrium equine; pregnancy; maternal recognition of pregnancy; embryo; non-coding RNA; endometrium
Show Figures

Figure 1

MDPI and ACS Style

Klohonatz, K.M.; Coleman, S.J.; Cameron, A.D.; Hess, A.M.; Reed, K.J.; Canovas, A.; Medrano, J.F.; Islas-Trejo, A.D.; Kalbfleisch, T.; Bouma, G.J.; Bruemmer, J.E. Non-Coding RNA Sequencing of Equine Endometrium During Maternal Recognition of Pregnancy. Genes 2019, 10, 821.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop