Identification of Long Non-Coding RNA-Associated Competing Endogenous RNA Network in the Differentiation of Chicken Preadipocytes
Abstract
:1. Introduction
2. Materials and Methods
2.1. Ethics Statement
2.2. Cell Culture and Induction
2.3. RNA Extraction and Library Construction
2.4. LncRNA Identification
2.5. miRNA Identification
2.6. Identification of Differentially Expressed Genes
2.7. Construction of LncRNAs-miRNAs-mRNAs ceRNA Regulatory Network
2.8. Functional Enrichment Analysis
2.9. Quantitative Reverse Transcription Polymerase Chain Reaction
2.10. Statistical Analysis
3. Results
3.1. Induction of Chicken Preadipocytes
3.2. Identification of Differentially Expressed lncRNAs, miRNAs, and mRNAs
3.3. Construction of ceRNA Network Related to Preadipocytes Differentiation
3.4. Functional Enrichment Analyses
3.5. Identification and RT-qPCR Validation of Crucial Genes
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Gene | Primer sequences |
---|---|
TOCNS_00055280 | GCAGAACGGATGGTGCCTTTGT |
GGCTGTCCTGGCTGAAGATGGA | |
TOCNS_00057242 | CCCTTCTCCGCTCACAGTCCTT |
CGCTTCGGCTGTAAACGTCCAT | |
TOCNS_00057272 | GGCTACCCGTCTCCTCCAAGAT |
GTGCCTCGTTACGCCTGATTGA | |
TOCNS_00026544 | ACGGAGATGCTGCGGTTATGC |
AACAAACCCAACCCGTTCCCAG | |
ENSGALT00000043224 | TCCAAGAAGGCGGTCACCAAGA |
GCACCTGCTTCAGCACCTTGT | |
FOXO6 | CAGCAGACCTGGACCTGGACAT |
CGCCGAGTCGAAGTTGAAGTCC | |
RASD1 | CCTCGTGTTCAGCCTGGACAAC |
CGGCACCTCGATGTTCTCCTTG | |
GPR133 | GTGAGCACCATCCGCAACCAA |
AAGGCACCGTTCCAGGACTGAA | |
JAM3 | GGAAGTCCTCCTCGCAGCAGTT |
AACAAGCCAGGTGCCCACTCT | |
CLDN1 | GCTGATTGCTTCCAACCAGGCT |
GCACACGGCTCTCCTTGTCTAC | |
TMEM123 | CGTACCACTCGAGAAGAGGC |
CAGCTGTGACAGGATGGGTT | |
gga-miR-6615-3p | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACTGTGGA |
CGCGTGGCACTGATGTGTTC | |
gga-miR-146a-3p | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACCTGAAG |
CGACCCATGGGGCTCAGTT | |
gga-miR-128-1-5p | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACTCTCAG |
GCGGGGCCGTAACACTGT | |
gga-miR-135a-5p | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACTCACAT |
CGCGTATGGCTTTTTATTCCT |
lncRNA | miRNA | mRNA |
---|---|---|
TCONS_00026544 | gga-miR-128-1-5p | RASD1 |
TCONS_00057272 | gga-miR-146a-3p | FOXO6 |
TCONS_00057242 | gga-miR-6615-3p | FOXO6 |
TCONS_00057242 | gga-miR-6615-3p | ENSGALT00000043224 |
TCONS_00040913 | gga-miR-6615-3p | ENSGALT00000043224 |
TCONS_00040913 | gga-miR-6615-3p | ENSGALT00000050730 |
TCONS_00038747 | gga-miR-6615-3p | ENSGALT00000050730 |
TCONS_00038748 | gga-miR-6615-3p | ENSGALT00000050730 |
TCONS_00055280 | gga-miR-135a-5p | JAM3 |
TCONS_00055280 | gga-miR-135a-5p | GPR133 |
TCONS_00055280 | gga-miR-135a-5p | CLDN1 |
TCONS_00055280 | gga-miR-135a-5p | TMEM123 |
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Chen, L.; Zhang, T.; Zhang, S.; Huang, J.; Zhang, G.; Xie, K.; Wang, J.; Wu, H.; Dai, G. Identification of Long Non-Coding RNA-Associated Competing Endogenous RNA Network in the Differentiation of Chicken Preadipocytes. Genes 2019, 10, 795. https://doi.org/10.3390/genes10100795
Chen L, Zhang T, Zhang S, Huang J, Zhang G, Xie K, Wang J, Wu H, Dai G. Identification of Long Non-Coding RNA-Associated Competing Endogenous RNA Network in the Differentiation of Chicken Preadipocytes. Genes. 2019; 10(10):795. https://doi.org/10.3390/genes10100795
Chicago/Turabian StyleChen, Lan, Tao Zhang, Shanshan Zhang, Jinxing Huang, Genxi Zhang, Kaizhou Xie, Jinyu Wang, Haiqing Wu, and Guojun Dai. 2019. "Identification of Long Non-Coding RNA-Associated Competing Endogenous RNA Network in the Differentiation of Chicken Preadipocytes" Genes 10, no. 10: 795. https://doi.org/10.3390/genes10100795