Overexpression of a Novel Cytochrome P450 Promotes Flavonoid Biosynthesis and Osmotic Stress Tolerance in Transgenic Arabidopsis
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Materials, Vectors, and Strains
2.2. De Novo Transcriptomic Assembly
2.3. Characterization and Phylogenetic Analysis
2.4. Extraction and cDNA Cloning of CtCYP82G24
2.5. Subcellular Compartmentalization of CtCYP82G24
2.6. Expression and Induction of Recombinant Protein
2.7. Vector Construction and Agrobacterium-Mediated Transformation of Arabidopsis
2.8. Metabolites Accumulation in Transgenic Plants
2.9. Quantitative RT-PCR Analysis
2.10. Statistical Analysis
3. Results
3.1. Discovery and Functional Annotation of CtCYP82G24
3.2. Phylogenetic and Conserved Domain Analysis
3.3. Cloning and Characterization of CtCYP82G24
3.4. Subcellular Localization of CtCYP82G24
3.5. Heterologous Expression and In Vitro Enzymatic Activity of CtCYP82G24
3.6. Identification of CtCYP82G24 Overexpressed Transgenic Lines of Arabidopsis
3.7. Expression Profiling of CtCYP82G24 and Metabolite Accumulation in Transgenic Plants under PEG-Induced Osmotic Stress
3.8. Overexpression of CtCYP82G24 Induced the Expression of Flavonoid Pathway Genes in Transgenic Arabidopsis
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Acknowledgments
Conflicts of Interest
Abbreviations
GFP | Green fluorescence protein |
KDa | Killo Dalton |
PI | Isoelectric point |
OD | Optical density |
PPIase | Peptidyl-prolyl cis-trans isomerase |
RRM | RNA recognition motif |
ZAT | Zinc transporter family |
MTPA2 | Metal tolerance protein |
SDS | Sodium Dodecyl Sulfate |
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Ahmad, N.; Jianyu, L.; Xu, T.; Noman, M.; Jameel, A.; Na, Y.; Yuanyuan, D.; Nan, W.; Xiaowei, L.; Fawei, W.; et al. Overexpression of a Novel Cytochrome P450 Promotes Flavonoid Biosynthesis and Osmotic Stress Tolerance in Transgenic Arabidopsis. Genes 2019, 10, 756. https://doi.org/10.3390/genes10100756
Ahmad N, Jianyu L, Xu T, Noman M, Jameel A, Na Y, Yuanyuan D, Nan W, Xiaowei L, Fawei W, et al. Overexpression of a Novel Cytochrome P450 Promotes Flavonoid Biosynthesis and Osmotic Stress Tolerance in Transgenic Arabidopsis. Genes. 2019; 10(10):756. https://doi.org/10.3390/genes10100756
Chicago/Turabian StyleAhmad, Naveed, Liu Jianyu, Tian Xu, Muhammad Noman, Aysha Jameel, Yao Na, Dong Yuanyuan, Wang Nan, Li Xiaowei, Wang Fawei, and et al. 2019. "Overexpression of a Novel Cytochrome P450 Promotes Flavonoid Biosynthesis and Osmotic Stress Tolerance in Transgenic Arabidopsis" Genes 10, no. 10: 756. https://doi.org/10.3390/genes10100756