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Article

High-Dimensional Single-Cell Quantitative Profiling of Skeletal Muscle Cell Population Dynamics during Regeneration

by
Lucia Lisa Petrilli
1,2,†,
Filomena Spada
1,†,
Alessandro Palma
1,
Alessio Reggio
1,
Marco Rosina
1,
Cesare Gargioli
1,
Luisa Castagnoli
1,
Claudia Fuoco
1,* and
Gianni Cesareni
1,3
1
Department of Biology, University of Rome “Tor Vergata”, 00133 Rome, Italy
2
Department of Onco-hematology, Gene and Cell Therapy—Bambino Gesù Children’s Hospital—IRCCS, 00146 Rome, Italy
3
Fondazione Santa Lucia Istituto di Ricovero e Cura a Carattere Scientifico (IRCCS), 00143 Rome, Italy
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to the study.
Cells 2020, 9(7), 1723; https://doi.org/10.3390/cells9071723
Submission received: 17 April 2020 / Revised: 3 June 2020 / Accepted: 14 July 2020 / Published: 18 July 2020
(This article belongs to the Special Issue Muscle Homeostasis and Regeneration: From Molecular Mechanisms to Therapeutic Opportunities)
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Abstract

The interstitial space surrounding the skeletal muscle fibers is populated by a variety of mononuclear cell types. Upon acute or chronic insult, these cell populations become activated and initiate finely-orchestrated crosstalk that promotes myofiber repair and regeneration. Mass cytometry is a powerful and highly multiplexed technique for profiling single-cells. Herein, it was used to dissect the dynamics of cell populations in the skeletal muscle in physiological and pathological conditions. Here, we characterized an antibody panel that could be used to identify most of the cell populations in the muscle interstitial space. By exploiting the mass cytometry resolution, we provided a comprehensive picture of the dynamics of the major cell populations that sensed and responded to acute damage in wild type mice and in a mouse model of Duchenne muscular dystrophy. In addition, we revealed the intrinsic heterogeneity of many of these cell populations.
Keywords: single-cell; mass cytometry; skeletal muscle regeneration; skeletal muscle homeostasis; fibro/adipogenic progenitors; myogenic progenitors; muscle populations single-cell; mass cytometry; skeletal muscle regeneration; skeletal muscle homeostasis; fibro/adipogenic progenitors; myogenic progenitors; muscle populations

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MDPI and ACS Style

Petrilli, L.L.; Spada, F.; Palma, A.; Reggio, A.; Rosina, M.; Gargioli, C.; Castagnoli, L.; Fuoco, C.; Cesareni, G. High-Dimensional Single-Cell Quantitative Profiling of Skeletal Muscle Cell Population Dynamics during Regeneration. Cells 2020, 9, 1723. https://doi.org/10.3390/cells9071723

AMA Style

Petrilli LL, Spada F, Palma A, Reggio A, Rosina M, Gargioli C, Castagnoli L, Fuoco C, Cesareni G. High-Dimensional Single-Cell Quantitative Profiling of Skeletal Muscle Cell Population Dynamics during Regeneration. Cells. 2020; 9(7):1723. https://doi.org/10.3390/cells9071723

Chicago/Turabian Style

Petrilli, Lucia Lisa, Filomena Spada, Alessandro Palma, Alessio Reggio, Marco Rosina, Cesare Gargioli, Luisa Castagnoli, Claudia Fuoco, and Gianni Cesareni. 2020. "High-Dimensional Single-Cell Quantitative Profiling of Skeletal Muscle Cell Population Dynamics during Regeneration" Cells 9, no. 7: 1723. https://doi.org/10.3390/cells9071723

APA Style

Petrilli, L. L., Spada, F., Palma, A., Reggio, A., Rosina, M., Gargioli, C., Castagnoli, L., Fuoco, C., & Cesareni, G. (2020). High-Dimensional Single-Cell Quantitative Profiling of Skeletal Muscle Cell Population Dynamics during Regeneration. Cells, 9(7), 1723. https://doi.org/10.3390/cells9071723

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