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Open AccessFeature PaperArticle

Glypican-1 Level Is Elevated in Extracellular Vesicles Released from MC38 Colon Adenocarcinoma Cells Overexpressing Snail

1
Institute of Medical Biology, Polish Academy of Sciences, 93-232 Lodz, Poland
2
CNRS UMR 7369, Matrice Extracellulaire et Dynamique Cellulaire (MEDyC), Laboratoire de Biochimie Médicale et Biologie Moléculaire, Université de Reims Champagne Ardenne, 51100 Reims, France
3
Laboratory of Microscopic Imaging and Specialized Biological Techniques, Faculty of Biology and Environmental Protection, University of Lodz, 90-237 Lodz, Poland
4
Department of Hematology, The Children’s Hospital of Philadelphia, Philadelphia, PA 19104, USA
*
Authors to whom correspondence should be addressed.
Cells 2020, 9(7), 1585; https://doi.org/10.3390/cells9071585
Received: 29 May 2020 / Revised: 25 June 2020 / Accepted: 26 June 2020 / Published: 30 June 2020
The transcription factor Snail triggers epithelial-to-mesenchymal transition (EMT), endowing cancer cells with invasive properties during tumor progression. Extracellular vesicles (EVs) released from cancer cells at various stages of cancer progression are known to influence the tumor pre-metastatic niche and metastatic potential. The aim of this study was to analyze the effect of Snail on murine colon adenocarcinoma cells (MC38 line) and on the characteristics of their EVs. Stable clones of Snail-overexpressing MC38 cells were investigated in vitro versus Mock cells. Increased expression of matrix metalloproteinase MMP-14 and augmented activity of MMP-9 and -14 were observed in Snail-MC38 cells. There was no change in the transcriptomic profile of proteoglycans in Snail-MC38 cells; however, the protein level of Glypican-1 (GPC1) was enhanced in EVs released from those cells. Our finding that GPC1 protein level was enhanced in EVs released from MC38 cells that overexpressed Snail and were in an early EMT stage might explain the specificity of the GPC1 biomarker in colon cancer diagnosis. Further, our data suggest that Snail, by changing the level of GPC1 on EVs released by colon cancer cells, may affect the generation of a distant premetastatic niche and metastatic organotropism in colon adenocarcinoma. View Full-Text
Keywords: colon adenocarcinoma; extracellular vesicles; epithelial-to-mesenchymal transition; Glypican-1 colon adenocarcinoma; extracellular vesicles; epithelial-to-mesenchymal transition; Glypican-1
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MDPI and ACS Style

Papiewska-Pająk, I.; Krzyżanowski, D.; Katela, M.; Rivet, R.; Michlewska, S.; Przygodzka, P.; Kowalska, M.A.; Brézillon, S. Glypican-1 Level Is Elevated in Extracellular Vesicles Released from MC38 Colon Adenocarcinoma Cells Overexpressing Snail. Cells 2020, 9, 1585.

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