Next Article in Journal
The Molecular Landscape of Hürthle Cell Thyroid Cancer Is Associated with Altered Mitochondrial Function—A Comprehensive Review
Previous Article in Journal
Role of Cyclin-Dependent Kinase 1 in Translational Regulation in the M-Phase
Previous Article in Special Issue
On the Host Side of the Hepatitis E Virus Life Cycle
Open AccessArticle

The RNA Replication Site of Tula Orthohantavirus Resides within a Remodelled Golgi Network

1
School of Molecular and Cellular Biology, University of Leeds, Leeds LS2 9JT, UK
2
National Infection Service, Public Health England, Porton Down, Salisbury SP4 0JG, UK
*
Author to whom correspondence should be addressed.
Cells 2020, 9(7), 1569; https://doi.org/10.3390/cells9071569
Received: 30 May 2020 / Revised: 22 June 2020 / Accepted: 23 June 2020 / Published: 27 June 2020
(This article belongs to the Special Issue Cell Biology of Viral Infections)
The family Hantaviridae within the Bunyavirales order comprises tri-segmented negative sense RNA viruses, many of which are rodent-borne emerging pathogens associated with fatal human disease. In contrast, hantavirus infection of corresponding rodent hosts results in inapparent or latent infections, which can be recapitulated in cultured cells that become persistently infected. In this study, we used Tula virus (TULV) to investigate the location of hantavirus replication during early, peak and persistent phases of infection, over a 30-day time course. Using immunofluorescent (IF) microscopy, we showed that the TULV nucleocapsid protein (NP) is distributed within both punctate and filamentous structures, with the latter increasing in size as the infection progresses. Transmission electron microscopy of TULV-infected cell sections revealed these filamentous structures comprised aligned clusters of filament bundles. The filamentous NP-associated structures increasingly co-localized with the Golgi and with the stress granule marker TIA-1 over the infection time course, suggesting a redistribution of these cellular organelles. The analysis of the intracellular distribution of TULV RNAs using fluorescent in-situ hybridization revealed that both genomic and mRNAs co-localized with Golgi-associated filamentous compartments that were positive for TIA. These results show that TULV induces a dramatic reorganization of the intracellular environment, including the establishment of TULV RNA synthesis factories in re-modelled Golgi compartments. View Full-Text
Keywords: hantavirus; Tula virus; replication; factory; RNA synthesis; Golgi; stress granules hantavirus; Tula virus; replication; factory; RNA synthesis; Golgi; stress granules
Show Figures

Figure 1

MDPI and ACS Style

Davies, K.A.; Chadwick, B.; Hewson, R.; Fontana, J.; Mankouri, J.; Barr, J.N. The RNA Replication Site of Tula Orthohantavirus Resides within a Remodelled Golgi Network. Cells 2020, 9, 1569.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop