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Article

Overlap of Peak Growth Activity and Peak IGF-1 to IGFBP Ratio: Delayed Increase of IGFBPs Versus IGF-1 in Serum as a Mechanism to Speed up and down Postnatal Weight Gain in Mice

1
Institute of Genome Biology, Leibniz-Institute for Farm Animal Biology (FBN), 18196 Dummerstorf, Germany
2
Institute of Genetics and Biometry, Leibniz-Institute for Farm Animal Biology (FBN), 18197 Dummerstorf, Germany
3
Versameb AG, 4057 Basel, Switzerland
4
Ligandis UG, 18276 Gülzow-Prüzen, Germany
*
Author to whom correspondence should be addressed.
Cells 2020, 9(6), 1516; https://doi.org/10.3390/cells9061516
Received: 14 April 2020 / Revised: 2 June 2020 / Accepted: 17 June 2020 / Published: 22 June 2020
(This article belongs to the Special Issue Insulin-Like Growth Factors in Development, Cancers and Aging)
Forced expression of insulin-like growth factor binding proteins (IGFBPs) in transgenic mice has clearly revealed inhibitory effects on somatic growth. However, by this approach, it cannot be solved if or how IGFBPs rule insulin-like growth factor (IGF)-dependent growth under normal conditions. In order to address this question, we have used growth-selected mouse models (obese and lean) and studied IGF-1 and IGFBPs in serum with respect to longitudinal growth activity in males and females compared with unselected controls. In mice of both genders, body weights were recorded and daily weight gains were calculated. Between 2 and 54 weeks of age, serum IGF-1 was determined by ELISA and intact IGFBP-2, -3 and -4 were quantified by Western ligand blotting. The molar ratio of IGF-1 to the sum of IGFBP-2 to -4 was calculated for all groups and plotted against the daily weight gain curve. Growth-selected mice are characterized by higher daily weight gains and extended periods of elevated growth activity if compared to matched unselected controls. Therefore, adult mice from the obese and lean groups can achieve more than twofold increased body weight in both genders (p < 0.001). Between 2 and 11 weeks of age, in obese and lean mice of both genders, serum IGF-1 concentrations are increased more prominently if compared to unselected controls (p < 0.001). Instead, substantial decreases of IGFBPs, particularly of IGFBP-2, are observed in males and females of all groups at the age of 2 to 4 weeks (p < 0.001). Due to the strong increase of IGF-1 but not of IGFBPs between two and four weeks of age, the ratio of IGF-1 to IGFBP-2 to -4 in serum significantly increased in all groups and genders (p < 0.05). Notably, the IGF-1 to IGFBP ratio was higher in male and female obese mice if compared to unselected controls (p < 0.05). View Full-Text
Keywords: longitudinal study; IGFBP; mouse models longitudinal study; IGFBP; mouse models
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MDPI and ACS Style

Walz, M.; Chau, L.; Walz, C.; Sawitzky, M.; Ohde, D.; Brenmoehl, J.; Tuchscherer, A.; Langhammer, M.; Metzger, F.; Höflich, C.; Hoeflich, A. Overlap of Peak Growth Activity and Peak IGF-1 to IGFBP Ratio: Delayed Increase of IGFBPs Versus IGF-1 in Serum as a Mechanism to Speed up and down Postnatal Weight Gain in Mice. Cells 2020, 9, 1516. https://doi.org/10.3390/cells9061516

AMA Style

Walz M, Chau L, Walz C, Sawitzky M, Ohde D, Brenmoehl J, Tuchscherer A, Langhammer M, Metzger F, Höflich C, Hoeflich A. Overlap of Peak Growth Activity and Peak IGF-1 to IGFBP Ratio: Delayed Increase of IGFBPs Versus IGF-1 in Serum as a Mechanism to Speed up and down Postnatal Weight Gain in Mice. Cells. 2020; 9(6):1516. https://doi.org/10.3390/cells9061516

Chicago/Turabian Style

Walz, Michael, Luong Chau, Christina Walz, Mandy Sawitzky, Daniela Ohde, Julia Brenmoehl, Armin Tuchscherer, Martina Langhammer, Friedrich Metzger, Christine Höflich, and Andreas Hoeflich. 2020. "Overlap of Peak Growth Activity and Peak IGF-1 to IGFBP Ratio: Delayed Increase of IGFBPs Versus IGF-1 in Serum as a Mechanism to Speed up and down Postnatal Weight Gain in Mice" Cells 9, no. 6: 1516. https://doi.org/10.3390/cells9061516

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