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Volume 9
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10.3390/cells9061444
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Communication

Tracking of Infused Mesenchymal Stem Cells in Injured Pulmonary Tissue in Atm-Deficient Mice

by
Patrick C. Baer
1,*,
Julia Sann
1,
Ruth Pia Duecker
2,
Evelyn Ullrich
3,4,5,
Helmut Geiger
1,
Peter Bader
3,
Stefan Zielen
2 and
Ralf Schubert
2,*
1
Division of Nephrology, Department of Internal Medicine III, University Hospital, Goethe-University, 60596 Frankfurt am Main, Germany
2
Division for Allergy, Pneumology and Cystic Fibrosis, Department for Children and Adolescents, University Hospital, Goethe-University, 60596 Frankfurt am Main, Germany
3
Division of Pediatric Stem Cell Transplantation and Immunology, Department for Children and Adolescents Medicine, University Hospital Frankfurt, Goethe University, 60596 Frankfurt am Main, Germany
4
Experimental Immunology, Department for Children and Adolescents Medicine, University Hospital Frankfurt, Goethe University, 60596 Frankfurt am Main, Germany
5
German Cancer Consortium (DKTK) partner site Frankfurt/Mainz, 60596 Frankfurt am Main, Germany
*
Authors to whom correspondence should be addressed.
Cells 2020, 9(6), 1444; https://doi.org/10.3390/cells9061444
Submission received: 7 May 2020 / Revised: 5 June 2020 / Accepted: 8 June 2020 / Published: 10 June 2020
(This article belongs to the Special Issue Adipose-Derived Stromal/Stem Cells)
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Abstract

Pulmonary failure is the main cause of morbidity and mortality in the human chromosomal instability syndrome Ataxia-telangiectasia (A-T). Major phenotypes include recurrent respiratory tract infections and bronchiectasis, aspiration, respiratory muscle abnormalities, interstitial lung disease, and pulmonary fibrosis. At present, no effective pulmonary therapy for A-T exists. Cell therapy using adipose-derived mesenchymal stromal/stem cells (ASCs) might be a promising approach for tissue regeneration. The aim of the present project was to investigate whether ASCs migrate into the injured lung parenchyma of Atm-deficient mice as an indication of incipient tissue damage during A-T. Therefore, ASCs isolated from luciferase transgenic mice (mASCs) were intravenously transplanted into Atm-deficient and wild-type mice. Retention kinetics of the cells were monitored using in vivo bioluminescence imaging (BLI) and completed by subsequent verification using quantitative real-time polymerase chain reaction (qRT-PCR). The in vivo imaging and the qPCR results demonstrated migration accompanied by a significantly longer retention time of transplanted mASCs in the lung parenchyma of Atm-deficient mice compared to wild type mice. In conclusion, our study suggests incipient damage in the lung parenchyma of Atm-deficient mice. In addition, our data further demonstrate that a combination of luciferase-based PCR together with BLI is a pivotal tool for tracking mASCs after transplantation in models of inflammatory lung diseases such as A-T.
Keywords: tracking; mesenchymal stromal/stem cells; bio imaging; bioluminescence; qRT-PCR; Ataxia telangiectasia; Atm tracking; mesenchymal stromal/stem cells; bio imaging; bioluminescence; qRT-PCR; Ataxia telangiectasia; Atm

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MDPI and ACS Style

Baer, P.C.; Sann, J.; Duecker, R.P.; Ullrich, E.; Geiger, H.; Bader, P.; Zielen, S.; Schubert, R. Tracking of Infused Mesenchymal Stem Cells in Injured Pulmonary Tissue in Atm-Deficient Mice. Cells 2020, 9, 1444. https://doi.org/10.3390/cells9061444

AMA Style

Baer PC, Sann J, Duecker RP, Ullrich E, Geiger H, Bader P, Zielen S, Schubert R. Tracking of Infused Mesenchymal Stem Cells in Injured Pulmonary Tissue in Atm-Deficient Mice. Cells. 2020; 9(6):1444. https://doi.org/10.3390/cells9061444

Chicago/Turabian Style

Baer, Patrick C., Julia Sann, Ruth Pia Duecker, Evelyn Ullrich, Helmut Geiger, Peter Bader, Stefan Zielen, and Ralf Schubert. 2020. "Tracking of Infused Mesenchymal Stem Cells in Injured Pulmonary Tissue in Atm-Deficient Mice" Cells 9, no. 6: 1444. https://doi.org/10.3390/cells9061444

APA Style

Baer, P. C., Sann, J., Duecker, R. P., Ullrich, E., Geiger, H., Bader, P., Zielen, S., & Schubert, R. (2020). Tracking of Infused Mesenchymal Stem Cells in Injured Pulmonary Tissue in Atm-Deficient Mice. Cells, 9(6), 1444. https://doi.org/10.3390/cells9061444

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