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Cells
Volume 9
Issue 4
10.3390/cells9041056
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Article

MiR-337-3p Promotes Adipocyte Browning by Inhibiting TWIST1

by
Indira G.C. Vonhögen
1,
Hamid el Azzouzi
1,2,
Servé Olieslagers
1,
Aliaksei Vasilevich
3,
Jan de Boer
3,
Francisco J. Tinahones
4,5,6,
Paula A. da Costa Martins
1,7,
Leon J. de Windt
1,* and
Mora Murri
4,5
1
Department of Molecular Genetics, Faculty of Sciences and Engineering, CARIM School for Cardiovascular Diseases, Faculty of Health, Medicine and Life Sciences, Maastricht University, 6200 MD Maastricht, The Netherlands
2
Department of Molecular Genetics, Erasmus University MC, 3015 GD Rotterdam, The Netherlands
3
BioInterface Science Group, Department of Biomedical Engineering and Institute for Complex Molecular Systems, Eindhoven University of Technology, 5600 MB Eindhoven, The Netherlands
4
Unidad de Gestión Clínica de Endocrinología y Nutrición, Instituto de Investigación Biomédica de Málaga (IBIMA), Hospital Clínico Virgen de la Victoria, 29010 Málaga, Spain
5
Centro de Investigación Biomédica en Red de Fisiopatología de la Obesidad y la Nutrición, CIBERObn, Instituto de Salud Carlos III, 28029 Madrid, Spain
6
Faculty of Medicine, University of Malaga, 29010 Malaga, Spain
7
Department of Physiology and Cardiothoracic Surgery, Faculty of Medicine, University of Porto, 4200-319 Porto, Portugal
*
Author to whom correspondence should be addressed.
Cells 2020, 9(4), 1056; https://doi.org/10.3390/cells9041056
Received: 28 March 2020 / Revised: 14 April 2020 / Accepted: 15 April 2020 / Published: 23 April 2020
(This article belongs to the Special Issue Molecular Mechanisms in Metabolic Disease)
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Abstract

The prevalence of metabolic syndrome (MetS) and obesity is an alarming health issue worldwide. Obesity is characterized by an excessive accumulation of white adipose tissue (WAT), and it is associated with diminished brown adipose tissue (BAT) activity. Twist1 acts as a negative feedback regulator of BAT metabolism. Therefore, targeting Twist1 could become a strategy for obesity and metabolic disease. Here, we have identified miR-337-3p as an upstream regulator of Twist1. Increased miR-337-3p expression paralleled decreased expression of TWIST1 in BAT compared to WAT. Overexpression of miR-337-3p in brown pre-adipocytes provoked a reduction in Twist1 expression that was accompanied by increased expression of brown/mitochondrial markers. Luciferase assays confirmed an interaction between the miR-337 seed sequence and Twist1 3′UTR. The inverse relationship between the expression of TWIST1 and miR-337 was finally validated in adipose tissue samples from non-MetS and MetS subjects that demonstrated a dysregulation of the miR-337-Twist1 molecular axis in MetS. The present study demonstrates that adipocyte miR-337-3p suppresses Twist1 repression and enhances the browning of adipocytes. View Full-Text
Keywords: brown adipose tissue; microRNA; mitochondria; obesity; metabolic syndrome brown adipose tissue; microRNA; mitochondria; obesity; metabolic syndrome
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited

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MDPI and ACS Style

Vonhögen, I.G.C.; el Azzouzi, H.; Olieslagers, S.; Vasilevich, A.; de Boer, J.; Tinahones, F.J.; da Costa Martins, P.A.; de Windt, L.J.; Murri, M. MiR-337-3p Promotes Adipocyte Browning by Inhibiting TWIST1. Cells 2020, 9, 1056. https://doi.org/10.3390/cells9041056

AMA Style

Vonhögen IGC, el Azzouzi H, Olieslagers S, Vasilevich A, de Boer J, Tinahones FJ, da Costa Martins PA, de Windt LJ, Murri M. MiR-337-3p Promotes Adipocyte Browning by Inhibiting TWIST1. Cells. 2020; 9(4):1056. https://doi.org/10.3390/cells9041056

Chicago/Turabian Style

Vonhögen, Indira G.C., Hamid el Azzouzi, Servé Olieslagers, Aliaksei Vasilevich, Jan de Boer, Francisco J. Tinahones, Paula A. da Costa Martins, Leon J. de Windt, and Mora Murri. 2020. "MiR-337-3p Promotes Adipocyte Browning by Inhibiting TWIST1" Cells 9, no. 4: 1056. https://doi.org/10.3390/cells9041056

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