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Open AccessArticle

Scalable Generation of Mesenchymal Stem Cells and Adipocytes from Human Pluripotent Stem Cells

1
Diabetes Research Center, Qatar Biomedical Research Institute (QBRI), Hamad Bin Khalifa University (HBKU), Qatar Foundation (QF), PO Box, Doha 34110, Qatar
2
Biological Sciences Program, Carnegie Mellon University in Qatar, Qatar Foundation, Education City, Doha 24866, Qatar
3
College of Health and Life Sciences, Hamad Bin Khalifa University (HBKU), Qatar Foundation (QF), Education City, Doha 34110, Qatar
*
Author to whom correspondence should be addressed.
Cells 2020, 9(3), 710; https://doi.org/10.3390/cells9030710
Received: 8 January 2020 / Revised: 4 March 2020 / Accepted: 9 March 2020 / Published: 13 March 2020
(This article belongs to the Special Issue Stem Cell-based Therapy and Disease Modeling)
Human pluripotent stem cells (hPSCs) can provide unlimited supply for mesenchymal stem cells (MSCs) and adipocytes that can be used for therapeutic applications. Here we developed a simple and highly efficient all-trans-retinoic acid (RA)-based method for generating an off-the-shelf and scalable number of human pluripotent stem cell (hPSC)-derived MSCs with enhanced adipogenic potential. We showed that short exposure of multiple hPSC lines (hESCs/hiPSCs) to 10 μM RA dramatically enhances embryoid body (EB) formation through regulation of genes activating signaling pathways associated with cell proliferation, survival and adhesion, among others. Disruption of cell adhesion induced the subsequent differentiation of the highly expanded RA-derived EB-forming cells into a pure population of multipotent MSCs (up to 1542-fold increase in comparison to RA-untreated counterparts). Interestingly, the RA-derived MSCs displayed enhanced differentiation potential into adipocytes. Thus, these findings present a novel RA-based approach for providing an unlimited source of MSCs and adipocytes that can be used for regenerative medicine, drug screening and disease modeling applications. View Full-Text
Keywords: retinoic acid; hPSCs; MSCs; apoptosis; proliferation; RNA-Seq; Adipogenic differentiation retinoic acid; hPSCs; MSCs; apoptosis; proliferation; RNA-Seq; Adipogenic differentiation
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Karam, M.; Younis, I.; Elareer, N.R.; Nasser, S.; Abdelalim, E.M. Scalable Generation of Mesenchymal Stem Cells and Adipocytes from Human Pluripotent Stem Cells. Cells 2020, 9, 710.

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