Chondrogenic Differentiation from Induced Pluripotent Stem Cells Using Non-Viral Minicircle Vectors
1
Catholic iPSC Research Center, College of Medicine, The Catholic University of Korea, Seoul 06591, Korea
2
Division of Rheumatology, Department of Internal Medicine, Seoul St. Mary’s Hospital, Institute of Medical Science, College of Medicine, The Catholic University of Korea, Seoul 06591, Korea
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Cells 2020, 9(3), 582; https://doi.org/10.3390/cells9030582
Received: 29 December 2019
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Revised: 18 February 2020
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Accepted: 27 February 2020
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Published: 1 March 2020
(This article belongs to the Special Issue Stem Cell-based Therapy and Disease Modeling)
Human degenerative cartilage has low regenerative potential. Chondrocyte transplantation offers a promising strategy for cartilage treatment and regeneration. Currently, chondrogenesis using human pluripotent stem cells (hiPSCs) is accomplished using human recombinant growth factors. Here, we differentiate hiPSCs into chondrogenic pellets using minicircle vectors. Minicircles are a non-viral gene delivery system that can produce growth factors without integration into the host genome. We generated minicircle vectors containing bone morphogenetic protein 2 (BMP2) and transforming growth factor beta 3 (TGFβ3) and delivered them to mesenchymal stem cell-like, hiPSC-derived outgrowth (OG) cells. Cell pellets generated using minicircle-transfected OG cells successfully differentiated into the chondrogenic lineage. The implanted minicircle-based chondrogenic pellets recovered the osteochondral defects in rat models. This work is a proof-of-concept study that describes the potential application of minicircle vectors in cartilage regeneration using hiPSCs.
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Keywords:
induced pluripotent stem cell; minicircle; chondrogenesis; BMP2; TGFβ3; growth factor; transfection
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MDPI and ACS Style
Rim, Y.A.; Nam, Y.; Park, N.; Jung, H.; Lee, K.; Lee, J.; Ju, J.H. Chondrogenic Differentiation from Induced Pluripotent Stem Cells Using Non-Viral Minicircle Vectors. Cells 2020, 9, 582. https://doi.org/10.3390/cells9030582
AMA Style
Rim YA, Nam Y, Park N, Jung H, Lee K, Lee J, Ju JH. Chondrogenic Differentiation from Induced Pluripotent Stem Cells Using Non-Viral Minicircle Vectors. Cells. 2020; 9(3):582. https://doi.org/10.3390/cells9030582
Chicago/Turabian StyleRim, Yeri A., Yoojun Nam, Narae Park, Hyerin Jung, Kijun Lee, Jennifer Lee, and Ji H. Ju. 2020. "Chondrogenic Differentiation from Induced Pluripotent Stem Cells Using Non-Viral Minicircle Vectors" Cells 9, no. 3: 582. https://doi.org/10.3390/cells9030582
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