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Article

The Drosophila RNA Helicase Belle (DDX3) Non-Autonomously Suppresses Germline Tumorigenesis Via Regulation of a Specific mRNA Set

Institute of Molecular Genetics, Russian Academy of Sciences, 2 Kurchatov Sq., 123182 Moscow, Russia
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Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Cells 2020, 9(3), 550; https://doi.org/10.3390/cells9030550
Received: 11 December 2019 / Revised: 20 February 2020 / Accepted: 25 February 2020 / Published: 26 February 2020
(This article belongs to the Special Issue Translational Regulation of Biological Processes)
DDX3 subfamily DEAD-box RNA helicases are essential developmental regulators of RNA metabolism in eukaryotes. belle, the single DDX3 ortholog in Drosophila, is required for fly viability, fertility, and germline stem cell maintenance. Belle is involved both in translational activation and repression of target mRNAs in different tissues; however, direct targets of Belle in the testes are essentially unknown. Here we showed that belle RNAi knockdown in testis cyst cells caused a disruption of adhesion between germ and cyst cells and generation of tumor-like clusters of stem-like germ cells. Ectopic expression of β-integrin in cyst cells rescued early stages of spermatogenesis in belle knockdown testes, indicating that integrin adhesion complexes are required for the interaction between somatic and germ cells in a cyst. To address Belle functions in spermatogenesis in detail we performed cross-linking immunoprecipitation and sequencing (CLIP-seq) analysis and identified multiple mRNAs that interacted with Belle in the testes. The set of Belle targets includes transcripts of proteins that are essential for preventing the tumor-like clusters of germ cells and for sustaining spermatogenesis. By our hypothesis, failures in the translation of a number of mRNA targets additively contribute to developmental defects observed in the testes with belle knockdowns both in cyst cells and in the germline. View Full-Text
Keywords: DDX3 RNA helicase; tumorigenesis; Drosophila testes; germline stem cells; cyst cells; translational regulation; CLIP-seq analysis DDX3 RNA helicase; tumorigenesis; Drosophila testes; germline stem cells; cyst cells; translational regulation; CLIP-seq analysis
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MDPI and ACS Style

Kotov, A.A.; Godneeva, B.K.; Olenkina, O.M.; Adashev, V.E.; Trostnikov, M.V.; Olenina, L.V. The Drosophila RNA Helicase Belle (DDX3) Non-Autonomously Suppresses Germline Tumorigenesis Via Regulation of a Specific mRNA Set. Cells 2020, 9, 550. https://doi.org/10.3390/cells9030550

AMA Style

Kotov AA, Godneeva BK, Olenkina OM, Adashev VE, Trostnikov MV, Olenina LV. The Drosophila RNA Helicase Belle (DDX3) Non-Autonomously Suppresses Germline Tumorigenesis Via Regulation of a Specific mRNA Set. Cells. 2020; 9(3):550. https://doi.org/10.3390/cells9030550

Chicago/Turabian Style

Kotov, Alexei A., Baira K. Godneeva, Oxana M. Olenkina, Vladimir E. Adashev, Mikhail V. Trostnikov, and Ludmila V. Olenina. 2020. "The Drosophila RNA Helicase Belle (DDX3) Non-Autonomously Suppresses Germline Tumorigenesis Via Regulation of a Specific mRNA Set" Cells 9, no. 3: 550. https://doi.org/10.3390/cells9030550

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