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Open AccessArticle

CD4+FOXP3+ T Cells in Rheumatoid Arthritis Bone Marrow Are Partially Impaired

1
Department of Pathophysiology and Immunology, National Institute of Geriatrics, Rheumatology, and Rehabilitation, 02-637 Warsaw, Poland
2
Department of Pathology, National Institute of Geriatrics, Rheumatology, and Rehabilitation, 02-637 Warsaw, Poland
3
Department of Diagnostic Hematology, Institute of Hematology and Transfusion Medicine, 02-776 Warsaw, Poland
4
Department of Rheumoorthopaedic Surgery, National Institute of Geriatrics, Rheumatology, and Rehabilitation, 02-637 Warsaw, Poland
5
Clinical Department of Orthopedic and Traumatology of Locomotor System, Enfant-Jesus Clinical Hospital, 02-005 Warsaw, Poland
6
Department of Pharmacy, Kepler University Hospital, 4020 Linz, Austria
7
Pharmaceutical Outcomes Programme, British Columbia Children’s Hospital, Vancouver, BC V5Z 4H4, Canada
*
Author to whom correspondence should be addressed.
Cells 2020, 9(3), 549; https://doi.org/10.3390/cells9030549
Received: 13 December 2019 / Revised: 13 February 2020 / Accepted: 20 February 2020 / Published: 26 February 2020
(This article belongs to the Special Issue The Molecular and Cellular Basis for Rheumatoid Arthritis)
There is evolving evidence that dysregulation of immune homeostasis in the bone marrow (BM) adjacent to the inflamed joints is involved in the pathogenesis of. In this study, we are addressing the phenotype and function of regulatory T cells (Tregs) residing in the BM of patients with rheumatoid arthritis (RA) and osteoarthritis (OA). BM and peripheral blood samples were obtained from RA and OA patients undergoing hip replacement surgery. The number and phenotype of Tregs were analyzed by flow cytometry and immunohistochemistry. The function of Tregs was investigated ex vivo, addressing their suppressive activity on effector T cells. [3H]-Thymidine incorporation assay and specific enzyme-linked immunosorbent assay were used for quantification of cell proliferation and pro-inflammatory (TNF, IFN-γ) cytokine release, respectively. Significantly lower numbers of CD4+FOXP3+ T cells were found in the BM of patients with RA compared to control patients with OA. High expression of CD127 (IL-7α receptor) and relatively low expression of CXCR4 (receptor for stromal cell-derived factor CXCL12) are characteristics of the CD4+FOXP3+ cells residing in the BM of RA patients. The BM-resident Tregs of RA patients demonstrated a limited suppressive activity on the investigated immune response. Our results indicate that the reduced number and impaired functional properties of CD4+FOXP3+ T cells present in the BM of RA patients may favor the inflammatory process, which is observed in RA BM. View Full-Text
Keywords: bone marrow; CD4+FOXP3+; Treg cells; rheumatoid arthritis; immunosuppression bone marrow; CD4+FOXP3+; Treg cells; rheumatoid arthritis; immunosuppression
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MDPI and ACS Style

Massalska, M.; Radzikowska, A.; Kuca-Warnawin, E.; Plebanczyk, M.; Prochorec-Sobieszek, M.; Skalska, U.; Kurowska, W.; Maldyk, P.; Kontny, E.; Gober, H.-J.; Maslinski, W. CD4+FOXP3+ T Cells in Rheumatoid Arthritis Bone Marrow Are Partially Impaired. Cells 2020, 9, 549. https://doi.org/10.3390/cells9030549

AMA Style

Massalska M, Radzikowska A, Kuca-Warnawin E, Plebanczyk M, Prochorec-Sobieszek M, Skalska U, Kurowska W, Maldyk P, Kontny E, Gober H-J, Maslinski W. CD4+FOXP3+ T Cells in Rheumatoid Arthritis Bone Marrow Are Partially Impaired. Cells. 2020; 9(3):549. https://doi.org/10.3390/cells9030549

Chicago/Turabian Style

Massalska, Magdalena; Radzikowska, Anna; Kuca-Warnawin, Ewa; Plebanczyk, Magdalena; Prochorec-Sobieszek, Monika; Skalska, Urszula; Kurowska, Weronika; Maldyk, Pawel; Kontny, Ewa; Gober, Hans-Jürgen; Maslinski, Wlodzimierz. 2020. "CD4+FOXP3+ T Cells in Rheumatoid Arthritis Bone Marrow Are Partially Impaired" Cells 9, no. 3: 549. https://doi.org/10.3390/cells9030549

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