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Volume 9
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10.3390/cells9020494
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Article

TLR2 Signaling Pathway Combats Streptococcus uberis Infection by Inducing Mitochondrial Reactive Oxygen Species Production

by
Bin Li
1,†,
Zhixin Wan
1,†,
Zhenglei Wang
1,
Jiakun Zuo
1,2,
Yuanyuan Xu
1,
Xiangan Han
2,
Vanhnaseng Phouthapane
3 and
Jinfeng Miao
1,*
1
MOE Joint International Research Laboratory of Animal Health and Food Safty, Key Laboratory of Animal Physiology and Biochemistry, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China
2
Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, China
3
Biotechnology and Ecology Institute, Ministry of Science and Technology (MOST), Vientiane 22797, Lao PDR
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Cells 2020, 9(2), 494; https://doi.org/10.3390/cells9020494
Submission received: 6 January 2020 / Revised: 9 February 2020 / Accepted: 19 February 2020 / Published: 21 February 2020
(This article belongs to the Special Issue Innate-Acquired Linkage in Immunotherapy)
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Abstract

Mastitis caused by Streptococcus uberis (S. uberis) is a common and difficult-to-cure clinical disease in dairy cows. In this study, the role of Toll-like receptors (TLRs) and TLR-mediated signaling pathways in mastitis caused by S. uberis was investigated using mouse models and mammary epithelial cells (MECs). We used S. uberis to infect mammary glands of wild type, TLR2−/− and TLR4−/− mice and quantified the adaptor molecules in TLR signaling pathways, proinflammatory cytokines, tissue damage, and bacterial count. When compared with TLR4 deficiency, TLR2 deficiency induced more severe pathological changes through myeloid differentiation primary response 88 (MyD88)-mediated signaling pathways during S. uberis infection. In MECs, TLR2 detected S. uberis infection and induced mitochondrial reactive oxygen species (mROS) to assist host in controlling the secretion of inflammatory factors and the elimination of intracellular S. uberis. Our results demonstrated that TLR2-mediated mROS has a significant effect on S. uberis-induced host defense responses in mammary glands as well as in MECs.
Keywords: Streptococcus uberis; mastitis; TLRs signaling pathway; mROS; inflammation Streptococcus uberis; mastitis; TLRs signaling pathway; mROS; inflammation

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MDPI and ACS Style

Li, B.; Wan, Z.; Wang, Z.; Zuo, J.; Xu, Y.; Han, X.; Phouthapane, V.; Miao, J. TLR2 Signaling Pathway Combats Streptococcus uberis Infection by Inducing Mitochondrial Reactive Oxygen Species Production. Cells 2020, 9, 494. https://doi.org/10.3390/cells9020494

AMA Style

Li B, Wan Z, Wang Z, Zuo J, Xu Y, Han X, Phouthapane V, Miao J. TLR2 Signaling Pathway Combats Streptococcus uberis Infection by Inducing Mitochondrial Reactive Oxygen Species Production. Cells. 2020; 9(2):494. https://doi.org/10.3390/cells9020494

Chicago/Turabian Style

Li, Bin, Zhixin Wan, Zhenglei Wang, Jiakun Zuo, Yuanyuan Xu, Xiangan Han, Vanhnaseng Phouthapane, and Jinfeng Miao. 2020. "TLR2 Signaling Pathway Combats Streptococcus uberis Infection by Inducing Mitochondrial Reactive Oxygen Species Production" Cells 9, no. 2: 494. https://doi.org/10.3390/cells9020494

APA Style

Li, B., Wan, Z., Wang, Z., Zuo, J., Xu, Y., Han, X., Phouthapane, V., & Miao, J. (2020). TLR2 Signaling Pathway Combats Streptococcus uberis Infection by Inducing Mitochondrial Reactive Oxygen Species Production. Cells, 9(2), 494. https://doi.org/10.3390/cells9020494

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