Curcumin Derivative GT863 Inhibits Amyloid-Beta Production via Inhibition of Protein N-Glycosylation
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Cell Lines and Cell Culture
2.3. Cell Treatment
2.4. Assessment of Cell Viability
2.5. ELISA for Aβ
2.6. Preparation of Cell Samples and Immunoblotting
2.7. In Vitro Secretase Assay
2.8. Statistical Analysis
3. Results
3.1. GT863 was Identified as a Potent Inhibitor of Aβ40 and Aβ42 Production in CHO-APP Cells.
3.2. Treatment with GT863 for 48 h Reduced Aβ40 and Aβ42 Production and Increased C83 and C99 Levels in CHO-APP Cells
3.3. GT863 Did Not Inhibit γ-Secretase Activity in Vitro
3.4. GT863 Inhibited γ-Cleavage in γ-Ssecretase Substrate-Selective Fashion in CHO-APP/NotchΔE and SH-SY5Y Cells
3.5. GT863 Suppressed N-Glycosylation of Proteins Including Nicastrin
3.6. Inhibition of Mannosidase Suppressed Aβ Production Without Affecting NotchΔE Cleavage.
4. Discussion
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Urano, Y.; Takahachi, M.; Higashiura, R.; Fujiwara, H.; Funamoto, S.; Imai, S.; Futai, E.; Okuda, M.; Sugimoto, H.; Noguchi, N. Curcumin Derivative GT863 Inhibits Amyloid-Beta Production via Inhibition of Protein N-Glycosylation. Cells 2020, 9, 349. https://doi.org/10.3390/cells9020349
Urano Y, Takahachi M, Higashiura R, Fujiwara H, Funamoto S, Imai S, Futai E, Okuda M, Sugimoto H, Noguchi N. Curcumin Derivative GT863 Inhibits Amyloid-Beta Production via Inhibition of Protein N-Glycosylation. Cells. 2020; 9(2):349. https://doi.org/10.3390/cells9020349
Chicago/Turabian StyleUrano, Yasuomi, Mina Takahachi, Ryo Higashiura, Hitomi Fujiwara, Satoru Funamoto, So Imai, Eugene Futai, Michiaki Okuda, Hachiro Sugimoto, and Noriko Noguchi. 2020. "Curcumin Derivative GT863 Inhibits Amyloid-Beta Production via Inhibition of Protein N-Glycosylation" Cells 9, no. 2: 349. https://doi.org/10.3390/cells9020349