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Article
Peer-Review Record

Combinatory Treatment of Canavanine and Arginine Deprivation Efficiently Targets Human Glioblastoma Cells via Pleiotropic Mechanisms

Cells 2020, 9(10), 2217; https://doi.org/10.3390/cells9102217
by Olena Karatsai 1, Pavel Shliaha 2, Ole N. Jensen 2, Oleh Stasyk 3 and Maria Jolanta Rędowicz 1,*
Reviewer 1: Anonymous
Reviewer 2: Anonymous
Cells 2020, 9(10), 2217; https://doi.org/10.3390/cells9102217
Submission received: 28 August 2020 / Revised: 23 September 2020 / Accepted: 24 September 2020 / Published: 30 September 2020
(This article belongs to the Special Issue Molecular and Cellular Mechanisms of Cancers: Glioblastoma)

Round 1

Reviewer 1 Report

I read with interest and pleasure the manuscript submitted by Karatsai et al. It is a well structured paper with a great number of used methods and clearly exposed results.

A possible critical aspect may be represented by the fact that the administration of canavanine plus arginine deprivation may lead to a slight ER stress and to a weak activation of pro-apoptotic factors after 48 h of treatment in normal glial cells. However, it is also to consider that these cells are from rat brain and it would be interesting to know how it could be the behavior of normal human astrocytes. Anyways, the overall data presented are sufficient to affirm that the proposed co-treatment has a valid anti-GBM effect  and, likely, few negative effects on normal glia.

Author Response

Please see the attachment

Author Response File: Author Response.pdf

Reviewer 2 Report

Interesting experimental study regarding the metabolism of glioblastoma cells.

 

How could this be incorporated as a therapy?

Could you comment on similar therapy approaches?

 

 

 

Author Response

Please see the attachment

Author Response File: Author Response.pdf

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