Estradiol Induces Epithelial to Mesenchymal Transition of Human Glioblastoma Cells
Abstract
:1. Introduction
2. Materials and Methods
2.1. TCGA Data Analysis
2.2. Cell Cultures
2.3. Cell Morphology Analysis
2.4. RT-qPCR
2.5. Western Blot
2.6. Immunofluorescence
2.7. Migration Assay
2.8. Invasion Assay
2.9. Statistical Analysis
3. Results
3.1. Differential Expression of ERα and ERβ Subtypes in Human GBM-Derived Cells
3.2. Changes in Cell Morphology During E2-Induced EMT
3.3. E2-Induced Reorganization of Actin Filaments
3.4. E2 Regulated EMT Marker Expression
3.5. E2 Promoted Migration and Invasion of Human GBM-Derived Cells
3.6. ER-α Mediated E2 Effects on EMT
4. Discussion
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Parameter | Description | Image |
---|---|---|
Area | The area included in the polygon that defines the figure contour | |
Axis major | Major axis length of an imaginary ellipse surrounding figure | |
Axis minor | Minor axis length of an imaginary ellipse surrounding figure | |
Aspect | The ratio between the major and minor axis of an ellipse | |
Bound box height | Bounding box height of the figure | |
Bound box width | Bounding box width of the figure | |
Box XY | The ratio of width to height of bounding box | |
Circularity | The ratio of figure area to the diameter of a circle around it | |
Perimeter | Length of the region surrounding the figure |
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Hernández-Vega, A.M.; Del Moral-Morales, A.; Zamora-Sánchez, C.J.; Piña-Medina, A.G.; González-Arenas, A.; Camacho-Arroyo, I. Estradiol Induces Epithelial to Mesenchymal Transition of Human Glioblastoma Cells. Cells 2020, 9, 1930. https://doi.org/10.3390/cells9091930
Hernández-Vega AM, Del Moral-Morales A, Zamora-Sánchez CJ, Piña-Medina AG, González-Arenas A, Camacho-Arroyo I. Estradiol Induces Epithelial to Mesenchymal Transition of Human Glioblastoma Cells. Cells. 2020; 9(9):1930. https://doi.org/10.3390/cells9091930
Chicago/Turabian StyleHernández-Vega, Ana M., Aylin Del Moral-Morales, Carmen J. Zamora-Sánchez, Ana G. Piña-Medina, Aliesha González-Arenas, and Ignacio Camacho-Arroyo. 2020. "Estradiol Induces Epithelial to Mesenchymal Transition of Human Glioblastoma Cells" Cells 9, no. 9: 1930. https://doi.org/10.3390/cells9091930