Myeloid Krüppel-Like Factor 2 Critically Regulates K/BxN Serum-Induced Arthritis
Abstract
:1. Introduction
2. Materials and Methods
2.1. Isolation and Culture of Monocytes from Murine Bone Marrow
2.2. Isolation of Human Monocytes
2.3. Real Time RT-PCR Analysis
2.4. Osteoclast Differentiation and TRAP Staining
2.5. Osteoclast Immunostaining
2.6. Induction of Arthritis in Mice
2.7. Histological Assessment of Arthritis
2.8. Western Blotting
2.9. Immunohistochemistry for Human Samples
2.10. Overexpression and Knockdown of KLF2
2.11. Chromatin Immunoprecipitation (ChIP) and Quantitative RT-PCR
2.12. Statistical Analysis
3. Results
3.1. Effect of K/BxN Serum-Induced Arthritis in KLF2 Hemizygous Mice
3.2. Effect of KLF2 Hemizygosity on Osteoclast Differentiation and Maturation
3.3. Expression of Molecules in Bone Marrow after Induced Arthritis
3.4. Effect of Monocyte-Specific Conditional KLF2 Knock Out on K/BxN Serum-Induced Arthritic Pathogenesis
3.5. Effect of Monocyte-Specific KLF2 Deficiency on Osteoclast Differentiation and Function
3.6. Expression of KLF2 in Monocytes after Arthritis Development in Mice
3.7. Recruitment of Activated Monocytes in Human Arthritic Joints and Expression of Molecules in Peripheral Blood Monocytes
3.8. Mechanistic Regulation of MMP9 by KLF2 in Monocytes
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Das, M.; Deb, M.; Laha, D.; Joseph, M.; Kanji, S.; Aggarwal, R.; Iwenofu, O.H.; Pompili, V.J.; Jarjour, W.; Das, H. Myeloid Krüppel-Like Factor 2 Critically Regulates K/BxN Serum-Induced Arthritis. Cells 2019, 8, 908. https://doi.org/10.3390/cells8080908
Das M, Deb M, Laha D, Joseph M, Kanji S, Aggarwal R, Iwenofu OH, Pompili VJ, Jarjour W, Das H. Myeloid Krüppel-Like Factor 2 Critically Regulates K/BxN Serum-Induced Arthritis. Cells. 2019; 8(8):908. https://doi.org/10.3390/cells8080908
Chicago/Turabian StyleDas, Manjusri, Moonmoon Deb, Dipranjan Laha, Matthew Joseph, Suman Kanji, Reeva Aggarwal, O. Hans Iwenofu, Vincent J. Pompili, Wael Jarjour, and Hiranmoy Das. 2019. "Myeloid Krüppel-Like Factor 2 Critically Regulates K/BxN Serum-Induced Arthritis" Cells 8, no. 8: 908. https://doi.org/10.3390/cells8080908