Genome-Wide Identification and Characterization of Long Noncoding RNAs of Brown to White Adipose Tissue Transformation in Goats
Abstract
:1. Introduction
2. Material and Methods
2.1. Ethics Statement
2.2. Animal and Sample Collection
2.3. Histology Analysis
2.4. Immunohistochemistry
2.5. RNA Extraction, Library Construction, and Sequencing
2.6. Quality Control and Transcriptome Assembly
2.7. Identification and Expression Analysis of Potential lncRNA
2.8. Target Gene Prediction and Enrichment Analysis
2.9. Quantitative Real-Time PCR
3. Results
3.1. Characterization of the Transformation from BAT to WAT during Perirenal Fat Development
3.2. Identification and Characterization of lncRNA in Perirenal Fat at Three Stages
3.3. Identification of Differentially Expressed lncRNAs during the Transformation from Brown to White Adipose Tissue
3.4. The Cis-Regulated Target Genes of lncRNAs
3.5. Enrichment Analysis of Coexpressed Genes of lncRNAs
3.6. Validation of lncRNAs by qPCR
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Wang, L.; Yang, X.; Zhu, Y.; Zhan, S.; Chao, Z.; Zhong, T.; Guo, J.; Wang, Y.; Li, L.; Zhang, H. Genome-Wide Identification and Characterization of Long Noncoding RNAs of Brown to White Adipose Tissue Transformation in Goats. Cells 2019, 8, 904. https://doi.org/10.3390/cells8080904
Wang L, Yang X, Zhu Y, Zhan S, Chao Z, Zhong T, Guo J, Wang Y, Li L, Zhang H. Genome-Wide Identification and Characterization of Long Noncoding RNAs of Brown to White Adipose Tissue Transformation in Goats. Cells. 2019; 8(8):904. https://doi.org/10.3390/cells8080904
Chicago/Turabian StyleWang, Linjie, Xin Yang, Yuehua Zhu, Siyuan Zhan, Zhe Chao, Tao Zhong, Jiazhong Guo, Yan Wang, Li Li, and Hongping Zhang. 2019. "Genome-Wide Identification and Characterization of Long Noncoding RNAs of Brown to White Adipose Tissue Transformation in Goats" Cells 8, no. 8: 904. https://doi.org/10.3390/cells8080904