Physical Exercise Affects Adipose Tissue Profile and Prevents Arterial Thrombosis in BDNF Val66Met Mice
Abstract
:1. Introduction
2. Materials and Methods
2.1. Mice
2.2. Voluntary Physical Exercise (PE) Protocol
2.3. Arterial Thrombosis Model
2.4. Whole Blood Counts
2.5. Platelet–Leukocyte Aggregate Analysis
2.6. Cell Culture, Treatment, and Differentiation
2.7. Adipogenesis Evaluation by Flow Cytometry and Oil-Red-O
2.8. Quantitative Real-Time Polymerase Chain Reaction (RT-qPCR)
2.9. Adipose Tissue Histology and Quantification of Adipocyte Size and Number
2.10. Statistical Analysis
3. Results
3.1. Characterization of the White Adipose Tissue Depots in BDNFMet/Met Mice
3.2. Evaluation of the Role of Mutant BDNF Val66Met Protein on Adipogenesis
3.3. Effect of Physical Exercise (PE) on Adipose Tissue Phenotype of BDNF Val66Met Mice
3.4. Effect of Physical Exercise (PE) on the Pro-Thrombotic Phenotype in BDNFMet/Met Mice
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Sandrini, L.; Ieraci, A.; Amadio, P.; Zarà, M.; Mitro, N.; Lee, F.S.; Tremoli, E.; Barbieri, S.S. Physical Exercise Affects Adipose Tissue Profile and Prevents Arterial Thrombosis in BDNF Val66Met Mice. Cells 2019, 8, 875. https://doi.org/10.3390/cells8080875
Sandrini L, Ieraci A, Amadio P, Zarà M, Mitro N, Lee FS, Tremoli E, Barbieri SS. Physical Exercise Affects Adipose Tissue Profile and Prevents Arterial Thrombosis in BDNF Val66Met Mice. Cells. 2019; 8(8):875. https://doi.org/10.3390/cells8080875
Chicago/Turabian StyleSandrini, Leonardo, Alessandro Ieraci, Patrizia Amadio, Marta Zarà, Nico Mitro, Francis S. Lee, Elena Tremoli, and Silvia Stella Barbieri. 2019. "Physical Exercise Affects Adipose Tissue Profile and Prevents Arterial Thrombosis in BDNF Val66Met Mice" Cells 8, no. 8: 875. https://doi.org/10.3390/cells8080875