Alzheimer’s Disease Research Using Human Microglia
Abstract
:1. Introduction
2. Development and Limitations of Methodology for Isolating Human Brain Microglia
2.1. Sources of Human Brain Tissue
2.2. Development of Microglial Isolation and Primary Culture Methods from Human Brains
2.3. Other Tissue Factors (Gray Matter Compared to White Matter)
2.4. Summary of the Methodology
3. Properties of Human Microglia in Culture
Human Microglia in Culture: How Much Do They Change Their Properties?
4. Experimental Findings on Alzheimer’s Disease Using Human Autopsy Microglia
4.1. In Vitro Responses of Human Microglia to Aβ
4.2. Human Microglial Responses to Aβ (1–42) Peptide
4.3. Human Microglial Responses: Differences Between AD and ND Microglia
4.4. Using Human Microglia to Study Inflammation Control Pathways Relevant to AD
4.5. Human Microglia and SNPs Associated with Altered Risk of AD
4.6. Aging Human Microglia
5. Enhancers of Proliferation, Cell Transformation and Microglial-Derived Induced Pluripotent Stem Cells (iPSCs)
5.1. Growth Factors for Inducing Cell Division in Human Microglia
5.2. Transformed Microglial Cell Lines
5.3. Human Microglia-Like Cells Produced from iPSCs
6. Conclusions
Funding
Conflicts of Interest
References
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Lue, L.-F.; Beach, T.G.; Walker, D.G. Alzheimer’s Disease Research Using Human Microglia. Cells 2019, 8, 838. https://doi.org/10.3390/cells8080838
Lue L-F, Beach TG, Walker DG. Alzheimer’s Disease Research Using Human Microglia. Cells. 2019; 8(8):838. https://doi.org/10.3390/cells8080838
Chicago/Turabian StyleLue, Lih-Fen, Thomas G. Beach, and Douglas G. Walker. 2019. "Alzheimer’s Disease Research Using Human Microglia" Cells 8, no. 8: 838. https://doi.org/10.3390/cells8080838