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Alzheimer’s Disease Research Using Human Microglia

Banner Sun Health Research Institute, Sun City, AZ 85351, USA
Neurodegenerative Disease Research Center and School of Life Sciences, Arizona State University, Tempe, AZ 84027, USA
Molecular Neuroscience Research Center, Shiga University of Medical Science, Otsu 520, Japan
Author to whom correspondence should be addressed.
Cells 2019, 8(8), 838;
Received: 4 June 2019 / Revised: 31 July 2019 / Accepted: 31 July 2019 / Published: 5 August 2019
(This article belongs to the Special Issue Microglia in Neurodegenerative Diseases)
Experimental studies of neuroinflammation in Alzheimer’s disease (AD) have mostly investigated microglia, the brain-resident macrophages. This review focused on human microglia obtained at rapid autopsies. Studies employing methods to isolate and culture human brain microglia in high purity for experimental studies were discussed. These methods were employed to isolate human microglia for investigation of a number of features of neuroinflammation, including activation phenotypes, neurotoxicity, responses to abnormal aggregated proteins such as amyloid beta, phagocytosis, and the effects of aging and disease on microglia cellular properties. In recent years, interest in human microglia and neuroinflammation has been renewed due to the identification of inflammation-related AD genetic risk factors, in particular the triggering receptor expressed on myeloid cells (TREM)-2. Because of the difficulties in developing effective treatments for AD, there has been a general need for greater understanding of the functions of microglia in normal and AD brains. While most experimental studies on neuroinflammation have employed rodent microglia, this review considered the role of human microglia in experimental studies. This review focused on the development of in vitro methodology for the culture of postmortem human microglia and the key findings obtained from experimental studies with these cells. View Full-Text
Keywords: neuroinflammation; microglia; cell culture; brain; amyloid; neurodegeneration; autopsy neuroinflammation; microglia; cell culture; brain; amyloid; neurodegeneration; autopsy
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Lue, L.-F.; Beach, T.G.; Walker, D.G. Alzheimer’s Disease Research Using Human Microglia. Cells 2019, 8, 838.

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