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Open AccessBrief Report

Wnt Receptor Frizzled-4 as a Marker for Isolation of Enteric Neural Progenitors in Human Children

1
Institute of Clinical Anatomy and Cell Analysis, University of Tübingen, 72074 Tübingen, Germany
2
Department of Pediatric Surgery, University Children’s Hospital, 72076 Tübingen, Germany
3
Department of Pediatric Surgery, University Children’s Hospital, 35043 Marburg, Germany
4
Department of Radiation Oncology, University of Tübingen, 72076 Tübingen, Germany
*
Author to whom correspondence should be addressed.
Cells 2019, 8(8), 792; https://doi.org/10.3390/cells8080792
Received: 28 June 2019 / Revised: 23 July 2019 / Accepted: 26 July 2019 / Published: 30 July 2019
(This article belongs to the Special Issue Wnt Signaling in Health and Diseases)
Identification and isolation of neural progenitor cells from the human enteric nervous system (ENS) is currently hampered by the lack of reliable, specific markers. Here, we define the Wnt-receptor frizzled-4 as a marker for the isolation of enteric neural progenitor cells derived from paediatric gut samples. We show that the Wnt-receptor frizzled-4 is expressed in the human colon and in Tunica muscularis-derived enterospheres. To obtain a purified culture, we carried out fluorescence-activated cell sorting (FACS) using PE-conjugated frizzled-4 antibodies. Frizzled-4positive cells gave rise to neurosphere-like bodies and ultimately differentiated into neurons as revealed by BrdU-proliferation assays and immunocytochemistry, whereas in frizzled-4negative cultures we did not detect any neuronal and glial cells. By using a patch-clamp approach, we also demonstrated the expression of functional sodium and potassium channels in frizzled-4positive cell cultures after differentiation in vitro. View Full-Text
Keywords: enteric nervous system; frizzled; Wnt; progenitor cells; stem cell marker; human stem cells; FACS enteric nervous system; frizzled; Wnt; progenitor cells; stem cell marker; human stem cells; FACS
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MDPI and ACS Style

Neckel, P.H.; Scharr, M.; Seid, K.; Nothelfer, K.; Fuchs, J.; Obermayr, F.; Hirt, B.; Huber, S.M.; Just, L. Wnt Receptor Frizzled-4 as a Marker for Isolation of Enteric Neural Progenitors in Human Children. Cells 2019, 8, 792.

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