NK Cell Induced T Cell Anergy Depends on GRAIL Expression
Abstract
:1. Introduction
2. Material and Methods
2.1. Animals
2.2. Reagents
2.3. In Vitro Preparation of HINT1/Hsp70 Complexes
2.4. EAE Induction and Pretreatment with HINT1/Hsp70 Complex
2.5. Magnetic Beads and FACS Cell Sorting
2.6. Flow Cytometry
2.7. CD4+ T Cell Proliferation Assay
2.8. In Vitro Cell Cocultures
2.9. Cell Transfection with siRNA or pcDNA Vector
2.10. Cell Cytotoxicity
2.11. Gel Electrophoresis and Western Blot
2.12. qRT-PCR
2.13. GRAIL Vector Construction
2.14. Statistical Methods
3. Results
3.1. EAE Inhibition Induced by HINT1/Hsp70 Depends on NK Cells
3.2. GRAIL Expression in CD4+ T Cells Is Indispensable for Inhibition of CD4+ T Cells Proliferation
3.3. NK Cell Inhibitory Effect Depends on GRAIL Expression
4. Discussion
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Galazka, G.; Domowicz, M.; Ewiak-Paszynska, A.; Jurewicz, A. NK Cell Induced T Cell Anergy Depends on GRAIL Expression. Cells 2019, 8, 790. https://doi.org/10.3390/cells8080790
Galazka G, Domowicz M, Ewiak-Paszynska A, Jurewicz A. NK Cell Induced T Cell Anergy Depends on GRAIL Expression. Cells. 2019; 8(8):790. https://doi.org/10.3390/cells8080790
Chicago/Turabian StyleGalazka, Grazyna, Malgorzata Domowicz, Alicja Ewiak-Paszynska, and Anna Jurewicz. 2019. "NK Cell Induced T Cell Anergy Depends on GRAIL Expression" Cells 8, no. 8: 790. https://doi.org/10.3390/cells8080790