Next Article in Journal
Differential Inhibition of Target Gene Expression by Human microRNAs
Next Article in Special Issue
The Molecular Basis for Remyelination Failure in Multiple Sclerosis
Previous Article in Journal
A Ciliary View of the Immunological Synapse
Previous Article in Special Issue
Naturally Occurring Nervonic Acid Ester Improves Myelin Synthesis by Human Oligodendrocytes
Open AccessBrief Report

NK Cell Induced T Cell Anergy Depends on GRAIL Expression

Department of Neurology, Medical University of Lodz, 22 Kopcinskiego str, 90–153 Lodz, Poland
*
Author to whom correspondence should be addressed.
Cells 2019, 8(8), 790; https://doi.org/10.3390/cells8080790
Received: 31 May 2019 / Revised: 22 July 2019 / Accepted: 22 July 2019 / Published: 29 July 2019
(This article belongs to the Special Issue The Molecular and Cellular Basis for Multiple Sclerosis)
NK cells (natural killer cells) being a part of the innate immune system have been shown to be involved in immunoregulation of autoimmune diseases. Previously we have shown that HINT1/Hsp70 treatment induced regulatory NK cells ameliorating experimental autoimmune encephalomyelitis (EAE) course and CD4+ T cells proliferation. NK cells were isolated from mice treated with HINT1/Hsp70 and co-cultured with proteolipid protein (PLP)-stimulated CD4+ T cells isolated from EAE mice. Cell proliferation was assessed by thymidine uptake, cytotoxicity by lactate dehydrogenase (LDH) release assay and fluorescence activated cell sorting (FACS) analysis, protein expression by Western blot, mRNA by quantitative RT-PCR. Gene related to anergy in lymphocytes (GRAIL) expression was downregulated by specific siRNA and GRAIL overexpression was induced by pcDNA-GRAIL transfection. HINT1/Hsp70 pretreatment of EAE SJL/J mice ameliorated EAE course, suppressed PLP-induced T cell proliferation by enhancing T cell expression of GRAIL as GRAIL downregulation restored T cell proliferation. HINT1/Hsp70 treatment induced immunoregulatory NK cells which inhibited PLP-stimulated T cell proliferation not depending on T cell necrosis and apoptosis. This immunoregulatory NK cell function depended on NK cell expression of GRAIL as GRAIL downregulation diminished inhibition of NK cell suppression of T cell proliferation. Similarly GRAIL overexpression in NK cells induced their regulatory function. HINT1/Hsp70 treatment generated regulatory NK cells characterized by expression of GRAIL. View Full-Text
Keywords: multiple sclerosis; experimental autoimmune encephalomyelitis; NK cell; anergy; GRAIL multiple sclerosis; experimental autoimmune encephalomyelitis; NK cell; anergy; GRAIL
Show Figures

Figure 1

MDPI and ACS Style

Galazka, G.; Domowicz, M.; Ewiak-Paszynska, A.; Jurewicz, A. NK Cell Induced T Cell Anergy Depends on GRAIL Expression. Cells 2019, 8, 790.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop