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Open AccessArticle

FZD10 Carried by Exosomes Sustains Cancer Cell Proliferation

1
Personalized Medicine Laboratory, National Institute of Gastroenterology “S. De Bellis”, Via Turi 27, Castellana Grotte, 70013 Bari, Italy
2
Institute for Chemical and Physical Processes (IPCF)-CNR SS Bari, Via Orabona 4, 70126 Bari, Italy
3
Dipartimento di Chimica, Università degli Studi di Bari Aldo Moro, Via Orabona 4, 70126 Bari, Italy
4
Laboratory of Clinical Biochemistry, National Institute of Gastroenterology “S. De Bellis”, Via Turi 27, Castellana Grotte, 70013 Bari, Italy
5
Dipartimento di Farmacia, Scienze del Farmaco, Università degli Studi di Bari Aldo Moro, Via Orabona 4, 70126 Bari, Italy
6
National Institute of Gastroenterology “S. De Bellis”, Scientific Direction, Via Turi 27, Castellana Grotte, 70013 Bari, Italy
*
Authors to whom correspondence should be addressed.
Cells 2019, 8(8), 777; https://doi.org/10.3390/cells8080777
Received: 8 July 2019 / Revised: 21 July 2019 / Accepted: 23 July 2019 / Published: 25 July 2019
(This article belongs to the Section Cell Signaling and Regulated Cell Death)
Extracellular vesicles (EVs) are involved in intercellular communication during carcinogenesis, and cancer cells are able to secrete EVs, in particular exosomes containing molecules, that can be transferred to recipient cells to induce pathological processes and significant modifications, as metastasis, increase of proliferation, and carcinogenesis evolution. FZD proteins, a family of receptors comprised in the Wnt signaling pathway, play an important role in carcinogenesis of the gastroenteric tract. Here, a still unknown role of Frizzled 10 (FZD10) protein was identified. In particular, the presence of FZD10 and FZD10-mRNA in exosomes extracted from culture medium of the untreated colorectal, gastric, hepatic, and cholangio cancer cell lines, was detected. A substantial reduction in the FZD10 and FZD10-mRNA level was achieved in FZD10-mRNA silenced cells and in their corresponding exosomes. Concomitantly, a significant decrease in viability of the silenced cells compared to their respective controls was observed. Notably, the incubation of silenced cells with the exosomes extracted from culture medium of the same untreated cells promoted the restoration of the cell viability and, also, of the FZD10 and FZD10-mRNA level, thus indicating that the FZD10 and FZD10-mRNA delivering exosomes may be potential messengers of cancer reactivation and play an active role in long-distance metastatization. View Full-Text
Keywords: colorectal cancer cells; gastric cancer cells; cholangiocarcinoma cells; hepatocarcinoma cells; exosomes; FZD10 protein; FZD10-mRNA; FZD10-mRNA silenced cells; cell proliferation colorectal cancer cells; gastric cancer cells; cholangiocarcinoma cells; hepatocarcinoma cells; exosomes; FZD10 protein; FZD10-mRNA; FZD10-mRNA silenced cells; cell proliferation
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Scavo, M.P.; Depalo, N.; Rizzi, F.; Ingrosso, C.; Fanizza, E.; Chieti, A.; Messa, C.; Denora, N.; Laquintana, V.; Striccoli, M.; Curri, M.L.; Giannelli, G. FZD10 Carried by Exosomes Sustains Cancer Cell Proliferation. Cells 2019, 8, 777.

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