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Open AccessArticle

Mitochondrial Transcriptome Control and Intercompartment Cross-Talk During Plant Development

1
Institute of Plant Molecular Biology (IBMP), CNRS and University of Strasbourg, 12 rue du Général Zimmer, 67084 Strasbourg, France
2
Centre of Agricultural Biochemistry and Biotechnology (CABB), University of Agriculture, Faisalabad 38000, Pakistan
3
Institute of Plant Sciences Paris-Saclay IPS2, CNRS, INRA, Université Paris-Sud, Université Evry, Université Paris-Saclay, Paris Diderot, Sorbonne Paris-Cité, 91405 Orsay, France
4
Institute of Bioorganic Chemistry, Polish Academy of Sciences, Ul. Z. Noskowskiego 12/14, 61-704 Poznan, Poland
5
Department of Computational Biology, Institute of Molecular Biology and Biotechnology, A. Mickiewicz University Poznan, Ul. Umultowska 89, 61-614 Poznan, Poland
6
NanoBioMedical Centre of the Adam Mickiewicz University, Umultowska 85, 61614 Poznan, Poland
*
Author to whom correspondence should be addressed.
These authors contributed equally to this project.
Cells 2019, 8(6), 583; https://doi.org/10.3390/cells8060583
Received: 21 March 2019 / Revised: 3 June 2019 / Accepted: 11 June 2019 / Published: 13 June 2019
(This article belongs to the Special Issue Mitochondrial Genetics)
We address here organellar genetic regulation and intercompartment genome coordination. We developed earlier a strategy relying on a tRNA-like shuttle to mediate import of nuclear transgene-encoded custom RNAs into mitochondria in plants. In the present work, we used this strategy to drive trans-cleaving hammerhead ribozymes into the organelles, to knock down specific mitochondrial RNAs and analyze the regulatory impact. In a similar approach, the tRNA mimic was used to import into mitochondria in Arabidopsis thaliana the orf77, an RNA associated with cytoplasmic male sterility in maize and possessing sequence identities with the atp9 mitochondrial RNA. In both cases, inducible expression of the transgenes allowed to characterise early regulation and signaling responses triggered by these respective manipulations of the organellar transcriptome. The results imply that the mitochondrial transcriptome is tightly controlled by a “buffering” mechanism at the early and intermediate stages of plant development, a control that is released at later stages. On the other hand, high throughput analyses showed that knocking down a specific mitochondrial mRNA triggered a retrograde signaling and an anterograde nuclear transcriptome response involving a series of transcription factor genes and small RNAs. Our results strongly support transcriptome coordination mechanisms within the organelles and between the organelles and the nucleus. View Full-Text
Keywords: anterograde regulation; cytoplasmic male sterility (CMS); plant mitochondria; retrograde regulation; ribozyme; RNA trafficking; signaling anterograde regulation; cytoplasmic male sterility (CMS); plant mitochondria; retrograde regulation; ribozyme; RNA trafficking; signaling
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Niazi, A.K.; Delannoy, E.; Iqbal, R.K.; Mileshina, D.; Val, R.; Gabryelska, M.; Wyszko, E.; Soubigou-Taconnat, L.; Szymanski, M.; Barciszewski, J.; Weber-Lotfi, F.; Gualberto, J.M.; Dietrich, A. Mitochondrial Transcriptome Control and Intercompartment Cross-Talk During Plant Development. Cells 2019, 8, 583.

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