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Grb7, a Critical Mediator of EGFR/ErbB Signaling, in Cancer Development and as a Potential Therapeutic Target

Analysis of Genetic Alterations in Tunisian Patients with Lung Adenocarcinoma

Biosciences Laboratory, Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori (IRST) IRCCS, 47014 Meldola, Italy
Laboratory of Human Molecular Genetics, Faculty of Medicine of Sfax, University of Sfax, Sfax 3029, Tunisia
Department of Respiratory and Sleep Diseases, CHU Hedi Chaker, Sfax 3029, Tunisia
Department of Pathology, CHU Habib Bourguiba, Sfax 3029, Tunisia
Laboratory of Anatomic Pathology, Sfax 3000, Tunisia
Author to whom correspondence should be addressed.
Cells 2019, 8(6), 514;
Received: 27 March 2019 / Revised: 23 May 2019 / Accepted: 26 May 2019 / Published: 28 May 2019
(This article belongs to the Special Issue Epidermal Growth Factor Receptor Signaling)
The identification of the mutations that drive lung cancer have furnished new targets for the treatment of non-small cell lung cancer (NSCLC) and led to the development of targeted therapies such as tyrosine kinase inhibitors that are used to combat the molecular changes promoting cancer progression. Furthermore, biomarkers identified from gene analysis can be used to detect early lung cancer, determine patient prognosis, and monitor response to therapy. In the present study we analyzed the molecular profile of seventy-three Tunisian patients with lung adenocarcinoma (LAD). Mutational analyses for EGFR and KRAS were performed using direct sequencing, immunohistochemistry or MassARRAY. Anaplastic lymphoma kinase (ALK) rearrangement was evaluated by immunohistochemistry using the D5F3 clone, and p53 expression was also assessed. The median age of patients at diagnosis was 61 years (range 23–82 years). Using different methodologies, EGFR mutations were found in 5.47% of patients and only exon 19 deletions “E746-A750 del” were detected. KRAS mutations were present in 9.58% of cases, while only one patient was ALK-positive. Moreover, abnormal immunostaining of p53 was detected in 56.16% of patients. In conclusion, the detected rates of EGFR and KRAS mutation and ALK rearrangement were lower than those found in European and Asian countries, whereas, abnormal p53 expression was slightly more frequent. Furthermore, given the small sample size of this study, a more comprehensive analysis of this patient set is warranted. View Full-Text
Keywords: molecular profile; EGFR; KRAS; ALK; p53; lung adenocarcinoma molecular profile; EGFR; KRAS; ALK; p53; lung adenocarcinoma
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MDPI and ACS Style

Dhieb, D.; Belguith, I.; Capelli, L.; Chiadini, E.; Canale, M.; Bravaccini, S.; Yangui, I.; Boudawara, O.; Jlidi, R.; Boudawara, T.; Calistri, D.; Ammar Keskes, L.; Ulivi, P. Analysis of Genetic Alterations in Tunisian Patients with Lung Adenocarcinoma. Cells 2019, 8, 514.

AMA Style

Dhieb D, Belguith I, Capelli L, Chiadini E, Canale M, Bravaccini S, Yangui I, Boudawara O, Jlidi R, Boudawara T, Calistri D, Ammar Keskes L, Ulivi P. Analysis of Genetic Alterations in Tunisian Patients with Lung Adenocarcinoma. Cells. 2019; 8(6):514.

Chicago/Turabian Style

Dhieb, Dhoha, Imen Belguith, Laura Capelli, Elisa Chiadini, Matteo Canale, Sara Bravaccini, Ilhem Yangui, Ons Boudawara, Rachid Jlidi, Tahya Boudawara, Daniele Calistri, Leila Ammar Keskes, and Paola Ulivi. 2019. "Analysis of Genetic Alterations in Tunisian Patients with Lung Adenocarcinoma" Cells 8, no. 6: 514.

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